Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
additional information | T4 DNA can adenylate the 5'-phosphorylated donor DNA substrate with a DNA template, ATP, and an acceptor strand that has a strategically chosen C-T acceptor-template mismatch directly adjacent to the adenylation site, between 0.5 and 10 mM ATP the adenylation yield is high (about 60%), whereas the yield is lower outside of this range | Tequatrovirus T4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T4 DNA ligase | - |
Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Tequatrovirus T4 |