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Literature summary for 6.5.1.1 extracted from

  • Lu, S.; Li, Z; Wang, Z.; Ma, X.; Sheng, D.; Ni, J.; Shen, Y.
    Spatial subunit distribution and in vitro functions of the novel trimeric PCNA complex from Sulfolobus tokodaii (2008), Biochem. Biophys. Res. Commun., 376, 369-374.
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged DNA ligase I in Escherichia coli strain BL21(DE3) Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Sulfurisphaera tokodaii
PCNA inhibition of the DNA ligase I activity by proliferating cell nuclear antigen, PCNA, complexes StoPCNA. StoPCNA1 and StoPCNA3, overview Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Sulfurisphaera tokodaii

Organic Solvent Stability

Organic Solvent Comment Organism
formamide inactivation at about 50% Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q976G4
-
-
Sulfurisphaera tokodaii 7 Q976G4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DNA ligase I from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + DNA substrate S4
-
 Sulfurisphaera tokodaii AMP + diphosphate + ?
-
 ?
ATP + DNA substrate S4
-
 Sulfurisphaera tokodaii 7 AMP + diphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA ligase I
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Sulfurisphaera tokodaii