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Literature summary for 6.5.1.1 extracted from
- Mutational analysis of Chlorella virus DNA ligase: catalytic roles of domain I and motif VI (1998), Nucleic Acids Res., 26, 4618-4625.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Chlorella virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Cdel5 | mutant lacking the C-terminal pentapeptide HEEDR of motif VI, 1% of wild-type DNA ligase activity | Chlorella virus |
| D297A | 14% of wild-type DNA ligase activity | Chlorella virus |
| E295A | 90% of wild-type DNA ligase activity | Chlorella virus |
| E296A | 52% of wild-type DNA ligase activity | Chlorella virus |
| F286A | 13% of wild-type DNA ligase activity | Chlorella virus |
| F289A | 42% of wild-type DNA ligase activity | Chlorella virus |
| H294A | 92% of wild-type DNA ligase activity | Chlorella virus |
| P284A | 53% of wild-type DNA ligase activity | Chlorella virus |
| P287A | 59% of wild-type DNA ligase activity | Chlorella virus |
| R285A | 26% of wild-type DNA ligase activity | Chlorella virus |
| R293A | 30% of wild-type DNA ligase activity | Chlorella virus |
| R298A | 85% of wild-type DNA ligase activity | Chlorella virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Chlorella virus | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorella virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant DNA ligase | Chlorella virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Chlorella virus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | 298 amino acid Chlorella virus PBCV-1 ligase is the smallest eukaryotic DNA ligase known | Chlorella virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PBCV-1 DNA ligase | - |
Chlorella virus |
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Release 2023.1 (January 2023)
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