Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibition above 40 mM | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
nicked DNA | 80°C, pH 8.0 | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 9% of activity with Mg2+, maximal activity at 5 mM, inhibition above 40 mM | Thermococcus kodakarensis | |
Mg2+ | divalent cation required for activity, maximal activity at 15 mM | Thermococcus kodakarensis | |
Mn2+ | 65% of activity with Mg2+, maximal activity at 25 mM | Thermococcus kodakarensis | |
Sr2+ | 40% of activity with Mg2+, maximal activity at 25 mM | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermococcus kodakarensis | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermococcus kodakarensis | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | proper base pairing at the 3' side of the nick is necessary for efficient ligation | Thermococcus kodakarensis | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + nicked DNA | - |
Thermococcus kodakarensis | AMP + diphosphate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ligTK | - |
Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
nicked DNA | 80°C, pH 8.0 | Thermococcus kodakarensis |