Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000029 | - |
(deoxyribonucleotide)20 | 25°C, pH 7.5 | Neisseria meningitidis | |
0.0004 | - |
ATP | 25°C, pH 7.5 | Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | approx. 10% of activity with Mg2+ | Neisseria meningitidis | |
Co2+ | approx. 12% of activity with Mg2+ | Neisseria meningitidis | |
Mg2+ | absolutely required for activity, maximal activity at 2 mM | Neisseria meningitidis | |
Mn2+ | approx. 20% of activity with Mg2+ | Neisseria meningitidis | |
Ni2+ | approx. 18% of activity with Mg2+ | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | 32000 | analytical ultracentrifugation | Neisseria meningitidis |
31540 | - |
gel filtration after preincubation with Mg2+ and diphosphate | Neisseria meningitidis |
31548 | - |
1 * 31548, deduced from nucleotide sequence | Neisseria meningitidis |
31873 | - |
1 * 31873, mass spectroscopy | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)20 + (deoxyribonucleotide)20 | Neisseria meningitidis | sealing of a single nick in a 20mer DNA duplex, ADL is specific for nicked DNA and is not able to catalyze blunt end joining | AMP + diphosphate + (deoxyribonucleotide)40 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant his-tagged ADL, nickel-affinity chromatography | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)20 + (deoxyribonucleotide)20 | sealing of a single nick in a 20mer DNA duplex, ADL is specific for nicked DNA and is not able to catalyze blunt end joining | Neisseria meningitidis | AMP + diphosphate + (deoxyribonucleotide)40 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31548, deduced from nucleotide sequence | Neisseria meningitidis |
monomer | 1 * 31873, mass spectroscopy | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
ADL | - |
Neisseria meningitidis |
ATP-dependent DNA ligase | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
(deoxyribonucleotide)20 | 25°C, pH 7.5 | Neisseria meningitidis |