Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.1 extracted from

  • Doherty, A.J.; Ashford, S.R.; Subramanya, H.S.; Wigley, D.B.
    Bacteriophage T7 DNA ligase. Overexpression, purification, crystallization, and characterization (1996), J. Biol. Chem., 271, 11083-11089.
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia phage T7

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia phage T7

Inhibitors

Inhibitors Comment Organism Structure
AMPPNP nonhydrolyzable ATP analogue Escherichia phage T7
AMPPNP no inhibition Tequatrovirus T4
EDTA
-
 Escherichia phage T7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 ATP ATP-diphosphate exchange reaction Escherichia phage T7
0.006
-
 ATP dATP Escherichia phage T7
0.006
-
 ATP joinig reaction Escherichia phage T7

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 equilibrium sedimentation Escherichia phage T7

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
-
-
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia phage T7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m can ligate nicked, cohesive, and blunt-ended DNA fragments Escherichia phage T7 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
additional information ATP-diphosphate exchange reaction Tequatrovirus T4 ?
-
 ?
additional information ATP-diphosphate exchange reaction Escherichia phage T7 ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Tequatrovirus T4
monomer 1 * 41000, SDS-PAGE Escherichia phage T7