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Literature summary for 6.4.1.3 extracted from

  • Arabolaza, A.; Shillito, M.E.; Lin, T.W.; Diacovich, L.; Melgar, M.; Pham, H.; Amick, D.; Gramajo, H.; Tsai, S.C.
    Crystal structures and mutational analyses of acyl-CoA carboxylase beta subunit of Streptomyces coelicolor (2010), Biochemistry, 49, 7367-7376.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 lambda(DE3) cells Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, mutant enzymes D422V and D422Lwith 0.1 M Tris pH 6.5, 2.0 M (NH4)SO4, mutant enzyme D422N with 0.1 M Bis-Tris pH 6.8, 10% (w/v) MPD, 0.2 M (NH4)OAc, mutant enzyme D422C with 0.1 M Na citrate pH 5.6, 0.2 M (NH4)SO4, and mutant enzyme D422A with 0.1 M Bis-Tris pH 6.2, 20% (w/v) PEG3350, 0.2 M (NH4)SO4 Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
D422A mutant of the PccB subunit, shows strong preference for butyryl-CoA as substrate Streptomyces coelicolor
D422C mutant of the PccB subunit, shows strong preference for butyryl-CoA as substrate Streptomyces coelicolor
D422I mutant of the PccB subunit, accepts acetyl-CoA, propionyl-CoA, and butyrl-CoA as substrates, the latter two with lower Vmax/Km values as compared to the wild type enzyme Streptomyces coelicolor
D422L mutant of the PccB subunit, the mutants has narrowed down their substrate specificity, accepting only propionyl-CoA as its substrate Streptomyces coelicolor
D422N mutant of the PccB subunit, the mutants has narrowed down their substrate specificity, accepting only propionyl-CoA as its substrate Streptomyces coelicolor
D422V mutant of the PccB subunit, accepts both propionyl-CoA and butyrl-CoA as substrates but with lower Vmax/Km values as compared to the wild type enzyme Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 butyryl-CoA mutant enzyme D422C, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.056
-
 propionyl-CoA mutant enzyme D422C, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.059
-
 butyryl-CoA mutant enzyme D422A, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.076
-
 propionyl-CoA wild type enzyme, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.077
-
 propionyl-CoA mutant enzyme D422V, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.104
-
 butyryl-CoA wild type enzyme, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.123
-
 propionyl-CoA mutant enzyme D422N, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.159
-
 propionyl-CoA mutant enzyme D422L, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.262
-
 propionyl-CoA mutant enzyme D422A, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.315
-
 propionyl-CoA mutant enzyme D422I, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.317
-
 butyryl-CoA mutant enzyme D422I, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.335
-
 acetyl-CoA mutant enzyme D422I, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor
0.383
-
 butyryl-CoA mutant enzyme D422V, in 100 mM potassium phosphate, pH 7.6, at 30°C Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9X4K7
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + CO2 the wild type enzyme shows no activity with acetyl-CoA, but mutant enzyme D422I does Streptomyces coelicolor ?
-
 ?
butyryl-CoA + CO2
-
 Streptomyces coelicolor ?
-
 ?
additional information the wild type enzyme shows no activity with acetyl-CoA Streptomyces coelicolor ?
-
 ?
propionyl-CoA + CO2
-
 Streptomyces coelicolor (S)-methylmalonyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
PCC
-
 Streptomyces coelicolor
PccB beta-subunit Streptomyces coelicolor
PccE epsilon-subunit Streptomyces coelicolor
Propionyl-CoA carboxylase
-
 Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
biotin
-
 Streptomyces coelicolor