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Literature summary for 6.4.1.3 extracted from

  • Jiang, H.; Rao, K.S.; Yee, V.C.; Kraus, J.P.
    Characterization of four variant forms of human propionyl-CoA carboxylase expressed in Escherichia coli (2005), J. Biol. Chem., 280, 27719-27727.
    View publication on PubMed
Search for more literature for 6.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
A497V polymorphism, 30% higher specific activity than wild-type enzyme Homo sapiens
E168K mutant enzyme shows 32.3% of the specific activity of purified wild-type enzyme Homo sapiens
R165W mutant enzyme shows 42.5% of the specific activity of purified wild-type enzyme Homo sapiens
R410W mutant enzyme shows 42.61% of the specific activity of purified wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 propanoyl-CoA 37°C, pH 8.0, mutant enzyme E168K Homo sapiens
0.33
-
 propanoyl-CoA 37°C, pH 8.0, mutant enzyme R165W Homo sapiens
0.41
-
 propanoyl-CoA 37°C, pH 8.0, wild-type enzyme Homo sapiens
0.54
-
 propanoyl-CoA 37°C, pH 8.0, mutant enzyme R410W Homo sapiens
0.59
-
 propanoyl-CoA 37°C, pH 8.0, mutant enzyme A497V Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
800000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.54
-
 purified mutant enzyme E168K Homo sapiens
9.93
-
 purified mutant enzyme R165W Homo sapiens
9.95
-
 purified mutant enzyme R410W Homo sapiens
23.35
-
 purified wild-type enzyme Homo sapiens
32.34
-
 purified mutant enzyme A497V Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + propanoyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha6beta6 Homo sapiens

Synonyms

Synonyms Comment Organism
PCC
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal denaturation is irreversible Homo sapiens
37
-
 30 min, wild-type enzyme is stable, mutant enzymes R165W, E168K and R410W lose 30% of their activity Homo sapiens
47
-
 30 min, wild-type enzyme is stable, mutant enzyme A497V loses 40% of its activity mutant enzymes R165W, E168K and R410W lose 85% of their activity Homo sapiens
58
-
 the wild-type enzyme undergoes a cooperative two-state transition between the native and denatured states with a Tm of 57.6°C Homo sapiens