Any feedback?
Literature summary for 6.4.1.3 extracted from
- Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation (2003), Eur. J. Biochem., 270, 736-744.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Metallosphaera sedula |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | weak inhibition | Metallosphaera sedula |  |
| CoA | activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA | Metallosphaera sedula | |
| malonyl-CoA | activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA | Metallosphaera sedula | |
| succinyl-CoA | activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA | Metallosphaera sedula | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
ATP | pH 7.5, 65°C | Metallosphaera sedula | |
| 0.06 | - |
acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
| 0.07 | - |
propanoyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
| 0.3 | - |
HCO3- | pH 7.5, 65°C | Metallosphaera sedula | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10% of the activity with Mg2+ | Metallosphaera sedula | |
| Co2+ | 40% of the activity with Mg2+ | Metallosphaera sedula | |
| Mg2+ | required for the formation of Mg-ATP complex, optimum concentration 5 mM at 5 mM ATP | Metallosphaera sedula | |
| Mn2+ | 10% of the activity with Mg2+ | Metallosphaera sedula | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18600 | - |
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) | Metallosphaera sedula |
| 57000 | - |
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) | Metallosphaera sedula |
| 560000 | - |
gel filtration | Metallosphaera sedula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + propanoyl-CoA + HCO3- | Chloroflexus aurantiacus | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | Metallosphaera sedula | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | Sulfuracidifex metallicus | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | Chloroflexus aurantiacus OK-70-fl / DSM 636 | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chloroflexus aurantiacus | - |
- |
- |
| Chloroflexus aurantiacus OK-70-fl / DSM 636 | - |
- |
- |
| Metallosphaera sedula | - |
- |
- |
| Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | - |
- |
- |
| Sulfuracidifex metallicus | - |
DSM 6482 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Metallosphaera sedula |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
purified enzyme, pH 7.5, 65°C | Metallosphaera sedula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | - |
Chloroflexus aurantiacus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | - |
Metallosphaera sedula | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | - |
Sulfuracidifex metallicus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Chloroflexus aurantiacus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Metallosphaera sedula | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Sulfuracidifex metallicus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | - |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| GTP + propanoyl-CoA + HCO3- | 10% of the activity with ATP | Metallosphaera sedula | GDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| GTP + propanoyl-CoA + HCO3- | 10% of the activity with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | GDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| UTP + propanoyl-CoA + HCO3- | 40% of the activity with ATP | Metallosphaera sedula | UDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
| UTP + propanoyl-CoA + HCO3- | 40% of the activity with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | UDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) | Metallosphaera sedula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-CoA/propionyl-CoA carboxylase | enzyme acts equally well on both substrates | Chloroflexus aurantiacus |
| acetyl-CoA/propionyl-CoA carboxylase | enzyme acts equally well on both substrates | Metallosphaera sedula |
| acetyl-CoA/propionyl-CoA carboxylase | enzyme acts equally well on both substrates | Sulfuracidifex metallicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Metallosphaera sedula |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 85 | - |
Metallosphaera sedula |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8 | propanoyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Metallosphaera sedula |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Metallosphaera sedula |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | bound to enzyme | Chloroflexus aurantiacus | |
| biotin | bound to enzyme | Metallosphaera sedula | |
| biotin | bound to enzyme | Sulfuracidifex metallicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
