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Literature summary for 6.4.1.3 extracted from

  • Kimura, Y.; Kojyo, T.; Kimura, I.; Sato, M.
    Propionyl-CoA carboxylase of Myxococcus xanthus: catalytic properties and function in developing cells (1998), Arch. Microbiol., 170, 179-184.
    View publication on PubMed

General Stability

General Stability Organism
crude enzyme is relatively unstable Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2
-
Myxococcus xanthus
CsCl
-
Myxococcus xanthus
CuCl2
-
Myxococcus xanthus
FeSO4
-
Myxococcus xanthus
KCl
-
Myxococcus xanthus
L-alanine
-
Myxococcus xanthus
L-phenylalanine
-
Myxococcus xanthus
L-serine
-
Myxococcus xanthus
L-tryptophan strong inhibitor Myxococcus xanthus
L-tyrosine
-
Myxococcus xanthus
L-valine
-
Myxococcus xanthus
LiCl
-
Myxococcus xanthus
NaCl
-
Myxococcus xanthus
RbCl
-
Myxococcus xanthus
ZnCl2
-
Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
propionyl-CoA pH 7.2, 30°C Myxococcus xanthus
0.036
-
ATP pH 7.2, 30°C Myxococcus xanthus
0.22
-
butyryl-CoA pH 7.2, 30°C Myxococcus xanthus
0.25
-
acetyl-CoA pH 7.2, 30°C Myxococcus xanthus
1.01
-
succinyl-CoA pH 7.2, 30°C Myxococcus xanthus
1.13
-
HCO3- pH 7.2, 30°C Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for the formation of an Mg-ATP complex, highest activity between 1.5-2.0 mM (at 1.0-1.3 mM ATP) Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) Myxococcus xanthus
56000
-
6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) Myxococcus xanthus
605000 615000 gel filtration Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + propanoyl-CoA + HCO3- Myxococcus xanthus key enzyme in catabolic pathways for long-chain fatty acids, polyketides and branched chain amino acids ADP + phosphate + (S)-methylmalonyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
maximum activity after 40 h of development
-

Purification (Commentary)

Purification (Comment) Organism
-
Myxococcus xanthus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.13
-
purified enzyme, pH 7.2, 30°C Myxococcus xanthus

Storage Stability

Storage Stability Organism
4°C, 20 mM phosphate buffer, pH 7.0, 2-4 weeks, no loss of activity Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3- EC 6.4.1.2 activity Myxococcus xanthus ADP + phosphate + malonyl-CoA
-
?
ATP + butyryl-CoA + HCO3-
-
Myxococcus xanthus ADP + phosphate + ?
-
?
ATP + propanoyl-CoA + HCO3-
-
Myxococcus xanthus ADP + phosphate + (S)-methylmalonyl-CoA
-
?
ATP + propanoyl-CoA + HCO3- key enzyme in catabolic pathways for long-chain fatty acids, polyketides and branched chain amino acids Myxococcus xanthus ADP + phosphate + (S)-methylmalonyl-CoA
-
?
ATP + succinyl-CoA + HCO3-
-
Myxococcus xanthus ADP + phosphate + ?
-
?
additional information methylmalonyl-CoA, malonyl-CoA, isobutyryl-CoA, and isovaleryl-CoA are not metabolized Myxococcus xanthus ?
-
?

Subunits

Subunits Comment Organism
multimer 6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Myxococcus xanthus

Cofactor

Cofactor Comment Organism Structure
biotin bound to alpha-subunit of the enzyme Myxococcus xanthus