General Stability | Organism |
---|---|
crude enzyme is relatively unstable | Myxococcus xanthus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | - |
Myxococcus xanthus | |
CsCl | - |
Myxococcus xanthus | |
CuCl2 | - |
Myxococcus xanthus | |
FeSO4 | - |
Myxococcus xanthus | |
KCl | - |
Myxococcus xanthus | |
L-alanine | - |
Myxococcus xanthus | |
L-phenylalanine | - |
Myxococcus xanthus | |
L-serine | - |
Myxococcus xanthus | |
L-tryptophan | strong inhibitor | Myxococcus xanthus | |
L-tyrosine | - |
Myxococcus xanthus | |
L-valine | - |
Myxococcus xanthus | |
LiCl | - |
Myxococcus xanthus | |
NaCl | - |
Myxococcus xanthus | |
RbCl | - |
Myxococcus xanthus | |
ZnCl2 | - |
Myxococcus xanthus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
propionyl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
0.036 | - |
ATP | pH 7.2, 30°C | Myxococcus xanthus | |
0.22 | - |
butyryl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
0.25 | - |
acetyl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
1.01 | - |
succinyl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
1.13 | - |
HCO3- | pH 7.2, 30°C | Myxococcus xanthus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for the formation of an Mg-ATP complex, highest activity between 1.5-2.0 mM (at 1.0-1.3 mM ATP) | Myxococcus xanthus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) | Myxococcus xanthus |
56000 | - |
6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) | Myxococcus xanthus |
605000 | 615000 | gel filtration | Myxococcus xanthus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + propanoyl-CoA + HCO3- | Myxococcus xanthus | key enzyme in catabolic pathways for long-chain fatty acids, polyketides and branched chain amino acids | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | - |
maximum activity after 40 h of development | - |
Purification (Comment) | Organism |
---|---|
- |
Myxococcus xanthus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.13 | - |
purified enzyme, pH 7.2, 30°C | Myxococcus xanthus |
Storage Stability | Organism |
---|---|
4°C, 20 mM phosphate buffer, pH 7.0, 2-4 weeks, no loss of activity | Myxococcus xanthus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Myxococcus xanthus | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + butyryl-CoA + HCO3- | - |
Myxococcus xanthus | ADP + phosphate + ? | - |
? | |
ATP + propanoyl-CoA + HCO3- | - |
Myxococcus xanthus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
ATP + propanoyl-CoA + HCO3- | key enzyme in catabolic pathways for long-chain fatty acids, polyketides and branched chain amino acids | Myxococcus xanthus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
ATP + succinyl-CoA + HCO3- | - |
Myxococcus xanthus | ADP + phosphate + ? | - |
? | |
additional information | methylmalonyl-CoA, malonyl-CoA, isobutyryl-CoA, and isovaleryl-CoA are not metabolized | Myxococcus xanthus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | 6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) | Myxococcus xanthus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Myxococcus xanthus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Myxococcus xanthus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | bound to alpha-subunit of the enzyme | Myxococcus xanthus |