Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rhizobium etli |
Protein Variants | Comment | Organism |
---|---|---|
R427K | more than 70% of mutant exist in the tetrameric form, Ka (activation value) for acetyl-CoA is 76fold higher compared to wild-type, kcat 32% of wild-type enzyme, Km (MgATP) 2.6fold higher compared to wild-type, kcat/Km value 12% of wild-type. Ka (activation value) for Mg2+ is 8fold higher compared to wild-type. Bicarbonate-dependent ATP cleavage activity: 0.4% of wild-type in the presence of acetyl-CoA/0.8% of wild-type in the absence of acetyl-CoA. ADP phosphorylation by carbamoyl phosphate: 21% of wild-type in the presence of acetyl-CoA. In the absence of acetyl-CoA activity slightly increased compared to wild-type. Mutant exhibits 6% biotin carboxylation rate of wild-type. residual activity at saturating concentrations of L-aspartate is 2fold greater than wild-type | Rhizobium etli |
R427S | more than 70% of mutant exist in the tetrameric form, Ka (activation value) for acetyl-CoA is 15fold higher compared to wild-type, kcat 41% of wild-type enzyme, Km (MgATP) 1.5fold higher compared to wild-type, kcat/Km value 26% of wild-type. Ka (activation value) for Mg2+ is 2fold higher compared to wild-type. Bicarbonate-dependent ATP cleavage activity: 1.1% of wild-type in the presence of acetyl-CoA/5.8% of wild-type in the absence of acetyl-CoA. ADP phosphorylation by carbamoyl phosphate: 17% of wild-type in the presence of acetyl-CoA. In the absence of acetyl-CoA activity similar to wild-type. Mutant exhibits 6% biotin carboxylation rate of wild-type | Rhizobium etli |
R472K | 45% of mutant exist in the tetrameric form, Ka (activation value) for acetyl-CoA is 252fold higher compared to wild-type, kcat 32% of wild-type enzyme, Km (MgATP) 6.7fold higher compared to wild-type, kcat/Km value 3% of wild-type. Ka (activation value) for Mg2+ is 37fold higher compared to wild-type. Bicarbonate-dependent ATP cleavage activity: 0.6% of wild-tpye in the presence of acetyl-CoA/0.9% of wild-type in the absence of acetyl-CoA. ADP phosphorylation by carbamoyl phosphate: 8% of wild-type in the presence of acetyl-CoA. In the absence of acetyl-CoA activity slightly increased compared to wild-type. Mutant exhibits 3% biotin carboxylation rate of wild-type | Rhizobium etli |
R472S | more than 70% of mutant exist in the tetrameric form, Ka (activation value) for acetyl-CoA is 203fold higher compared to wild-type, kcat 25% of wild-type enzyme, Km (MgATP) 8.7fold higher compared to wild-type, kcat/Km value 2% of wild-type. Ka (activation value) for Mg2+ is 30fold higher compared to wild-type. Bicarbonate-dependent ATP cleavage activity: 0.6% of wild-type in the presence of acetyl-CoA/1.2% of wild-type in the absence of acetyl-CoA. ADP phosphorylation by carbamoyl phosphate: 5% of wild-type in the presence of acetyl-CoA. In the absence of acetyl-CoA activity slightly increased compared to wild-type. Mutant exhibits 2% biotin carboxylation rate of wild-type | Rhizobium etli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-aspartate | - |
Rhizobium etli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | 1 | ATP | wild-type, pH 7.8, 30°C | Rhizobium etli | |
0.16 | - |
ATP | mutant R427S, pH 7.8, 30°C | Rhizobium etli | |
0.29 | - |
ATP | mutant R427K, pH 7.8, 30°C | Rhizobium etli | |
0.7 | - |
ATP | mutant R472K, pH 7.8, 30°C | Rhizobium etli | |
1 | - |
ATP | mutant R472S, pH 7.8, 30°C | Rhizobium etli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | Q2K340 | - |
- |
Rhizobium etli CFN 42 | Q2K340 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + carbamoylphosphate | reverse reaction is assessed by using substrate analogue carbamoylphosphate | Rhizobium etli | ? | - |
r | |
ADP + carbamoylphosphate | reverse reaction is assessed by using substrate analogue carbamoylphosphate | Rhizobium etli CFN 42 | ? | - |
r | |
ATP + pyruvate + HCO3- | - |
Rhizobium etli | ADP + phosphate + oxaloacetate | - |
r | |
ATP + pyruvate + HCO3- | - |
Rhizobium etli CFN 42 | ADP + phosphate + oxaloacetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Rhizobium etli |
Synonyms | Comment | Organism |
---|---|---|
pyruvate carboxylase | - |
Rhizobium etli |
RePC | - |
Rhizobium etli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhizobium etli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
ATP | mutant R472K, pH 7.8, 30°C | Rhizobium etli | |
2 | - |
ATP | mutant R472S, pH 7.8, 30°C | Rhizobium etli | |
3.4 | - |
ATP | mutant R427K, pH 7.8, 30°C | Rhizobium etli | |
4.2 | - |
ATP | mutant R427S, pH 7.8, 30°C | Rhizobium etli | |
10.9 | - |
ATP | wild-type, pH 7.8, 30°C | Rhizobium etli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rhizobium etli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | - |
Rhizobium etli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
L-aspartate | mutant R427K, pH 7.8, 30°C | Rhizobium etli | |
5 | - |
L-aspartate | wild-type, pH 7.8, 30°C | Rhizobium etli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
ATP | mutant R472S, pH 7.8, 30°C | Rhizobium etli | |
3 | - |
ATP | mutant R472K, pH 7.8, 30°C | Rhizobium etli | |
12 | - |
ATP | mutant R427K, pH 7.8, 30°C | Rhizobium etli | |
26 | - |
ATP | mutant R427S, pH 7.8, 30°C | Rhizobium etli | |
99 | - |
ATP | wild-type, pH 7.8, 30°C | Rhizobium etli |