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Literature summary for 6.4.1.1 extracted from

  • Hasan, N.M.; Longacre, M.J.; Stoker, S.W.; Boonsaen, T.; Jitrapakdee, S.; Kendrick, M.A.; Wallace, J.C.; MacDonald, M.J.
    Impaired anaplerosis and insulin secretion in insulinoma cells caused by small interfering RNA-mediated suppression of pyruvate carboxylase (2008), J. Biol. Chem., 283, 28048-28059.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression analysis of the enzyme in diverse wild-type and knockout insulinoma cell lines, overview Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information suppression by stable expression of siRNA causes impaired anaplerosis and insulin secretion in insulinoma cells, knockout in INS-1 832/13 cells, cell lines U6 and CHS, and in INS-1 832/13-derived cell lines PCX3, PC1971, PC1973, PC3064, and PC118. Insulin release in response to pyruvate alone, 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid plus glutamine, or methyl succinate plus beta-hydroxybutyrate is also decreased in the PC knockdown cells, phenotype, overview Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
phenylacetate weak inhibitor Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + HCO3- Rattus norvegicus
-
 ADP + phosphate + oxaloacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P52873
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreatic beta cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
 Rattus norvegicus ADP + phosphate + oxaloacetate
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus