Cloned (Comment) | Organism |
---|---|
coexpression of pyruvate carboxylase 1 isozyme (Pyc1) with an N-terminal myc tag, together with constructs encoding either the biotin carboxylase domain or the transcarboxylase-biotin carboxyl carrier domain, each with an N-terminal 9-histidine tag | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E40R | predominant form of mutant E40R is the monomer. Coexpression of mutant forms with wild type Pyc1 shows that mutations causes severe loss of interaction with wild type Pyc1 | Saccharomyces cerevisiae |
E433R | predominant form of mutant E433R is the monomer. Coexpression of mutant forms with wild type Pyc1 shows that mutations causes severe loss of interaction with wild type Pyc1 | Saccharomyces cerevisiae |
R36E | the R36E is much more susceptible to tetramer dissociation and inactivation than the wild type enzyme. Coexpression of mutant forms with wild type Pyc1 shows that the R36E mutation had no effect on the interaction of these subunits with those of wild type Pyc1 | Saccharomyces cerevisiae |
R36E/E433R | predominant form of mutant R36E/E433R is the monomer. Coexpression of mutant forms with wild type Pyc1 shows that mutations causes severe loss of interaction with wild type Pyc1 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P11154 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
tetramer | Glu40 and Glu433 play essential roles in subunit interactions | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Pyc1 | - |
Saccharomyces cerevisiae |
pyruvate carboxylase 1 | - |
Saccharomyces cerevisiae |