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Literature summary for 6.4.1.1 extracted from

  • Kim, S.H.; Lee, G.M.
    Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44) (2007), Appl. Microbiol. Biotechnol., 76, 659-665.
    View publication on PubMed

Application

Application Comment Organism
biotechnology FLAG-tagged human pyruvate carboxylase is introduced into a dihydrofolate-deficient CHO cell line DG44. Through the expression of the human pyruvate carboxylase enzyme, lactate formation in CHO cell culture can be efficiently reduced. This effect of expression of the human pyruvate carboxylase is observed not only in adherent batch culture using the serum-containing medium but in the serum-free suspension fed-batch culture as well, demonstrating its potential use to extend the culture longevity of CHO cell culture, which often shows a significant accumulation of lactate Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
FLAG-tagged human pyruvate carboxylase is introduced into a dihydrofolate-deficient CHO cell line DG44. Through the expression of the human pyruvate carboxylase enzyme, lactate formation in CHO cell culture can be efficiently reduced. This effect of expression of the human pyruvate carboxylase is observed not only in adherent batch culture using the serum-containing medium but in the serum-free suspension fed-batch culture as well, demonstrating its potential use to extend the culture longevity of CHO cell culture, which often shows a significant accumulation of lactate Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P11498
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Source Tissue

Source Tissue Comment Organism Textmining