Literature summary for 6.4.1.1 extracted from

Mukhopadhyay, B.; Purwantini, E.
Pyruvate carboxylase from Mycobacterium smegmatis: stabilization, rapid purification, molecular and biochemical characterization and regulation of the cellular level (2000), Biochim. Biophys. Acta, 1475, 191-206.

Search for more literature for 6.4.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
acetyl-CoA	dependent, half maximal activation at 0.014 mM	Mycolicibacterium smegmatis
butyryl-CoA	can replace acetyl-CoA with little efficiency	Mycolicibacterium smegmatis
propionyl-CoA	can replace acetyl-CoA with little efficiency	Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mycolicibacterium smegmatis

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Mycolicibacterium smegmatis
ATP	-	Mycolicibacterium smegmatis
Co2+	at excess concentrations	Mycolicibacterium smegmatis
L-aspartate	47% inhibition at 10 mM	Mycolicibacterium smegmatis
Mg2+	at excess concentrations	Mycolicibacterium smegmatis
Mn2+	at excess concentrations	Mycolicibacterium smegmatis
additional information	no inhibitors: glutamate and alpha-ketoglutarate	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	ATP	37°C and 45°C, pH 8, Mg2+ concentration higher than ATP concentration	Mycolicibacterium smegmatis
0.3	-	pyruvate	37°C, pH 8	Mycolicibacterium smegmatis
0.45	-	pyruvate	45°C, pH 8	Mycolicibacterium smegmatis
1.26	-	ATP	37°C, pH 8, Mg2+ concentration equal to that of ATP	Mycolicibacterium smegmatis
3	-	HCO3-	37°C, pH 8	Mycolicibacterium smegmatis
4.6	-	HCO3-	45°C, pH 8	Mycolicibacterium smegmatis
9.2	-	ATP	45°C, pH 8, Mg2+ concentration equal to that of ATP	Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	can replace Mg2+ with little efficiency	Mycolicibacterium smegmatis
Mg2+	requirement	Mycolicibacterium smegmatis
Mn2+	can replace Mg2+ with little efficiency	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	x * 120000, SDS-PAGE	Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + pyruvate + HCO3-	Mycolicibacterium smegmatis	anaplerotic enzyme	ADP + phosphate + oxaloacetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
150	-	-	Mycolicibacterium smegmatis

Storage Stability

Storage Stability	Organism
-70°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol	Mycolicibacterium smegmatis
4°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pyruvate + HCO3-	-	Mycolicibacterium smegmatis	ADP + phosphate + oxaloacetate	-	?
ATP + pyruvate + HCO3-	anaplerotic enzyme	Mycolicibacterium smegmatis	ADP + phosphate + oxaloacetate	-	?

Subunits

Subunits	Comment	Organism
multimer	x * 120000, SDS-PAGE	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
PC	-	Mycolicibacterium smegmatis
PYC	-	Mycolicibacterium smegmatis

Cofactor

Cofactor	Comment	Organism	Structure
biotin	-	Mycolicibacterium smegmatis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.24	-	ATP	45°C, pH 8	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)