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Literature summary for 6.4.1.1 extracted from
- Functional expression, purification, and characterization of recombinant human pyruvate carboxylase (1999), Biochem. Biophys. Res. Commun., 266, 512-517.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | allosteric activator, 50% of maximal activity at 0.013 mM for the recombinant enzyme and at 0.015 mM for the liver enzyme | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in 293T cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
ATP | 37°C, pH 7.8, liver enzyme | Homo sapiens | |
| 0.22 | - |
pyruvate | 37°C, pH 7.8, liver enzyme | Homo sapiens | |
| 0.23 | - |
pyruvate | 37°C, pH 7.8, recombinant enzyme | Homo sapiens | |
| 0.25 | - |
ATP | 37°C, pH 7.8, recombinant enzyme | Homo sapiens | |
| 1.75 | - |
HCO3- | 37°C, pH 7.8, recombinant enzyme | Homo sapiens | |
| 3.2 | - |
HCO3- | 37°C, pH 7.8, liver enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | Homo sapiens | - |
ADP + phosphate + oxaloacetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P11498 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme and enzyme from liver | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | - |
Homo sapiens | ADP + phosphate + oxaloacetate | - |
? | |
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