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Literature summary for 6.4.1.1 extracted from
- Acetyl-CoA-dependent pyruvate carboxylase from the photosynthetic bacterium Rhodobacter capsulatus: rapid and efficient purification using dye-ligand affinity chromatography (1995), Microbiology, 141, 2619-2628.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodobacter capsulatus |
General Stability
| General Stability | Organism |
|---|---|
| acetyl-CoA protects against thermal denaturation | Rhodobacter capsulatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Rhodobacter capsulatus |  |
| ADP | - |
Rhodobacter capsulatus | |
| Avidin | - |
Rhodobacter capsulatus | |
| L-Asp | - |
Rhodobacter capsulatus | |
| Mn2+ | inhibition above 1.25 mM, activation below | Rhodobacter capsulatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | completely dependent on divalent metal ion, Mg2+ or Mn2+ | Rhodobacter capsulatus | |
| Mn2+ | divalent metal ion required, Mg2+ or Mn2+. Mn2+ inhibits at concentrations above 1.25 mM | Rhodobacter capsulatus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
overexpressing strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| rapid dye-ligand affinity chromatography procedure | Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | - |
Rhodobacter capsulatus | ADP + phosphate + oxaloacetate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
acetyl-CoA protects against thermal inactivation | Rhodobacter capsulatus |
| 20 | - |
1 h, 75% inactivation | Rhodobacter capsulatus |
| 50 | - |
15 min, complete loss of activity | Rhodobacter capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activated by MnCl2 | Rhodobacter capsulatus |
| 8 | - |
activated by Mg2+, at a MgCl2 concentration double that of ATP | Rhodobacter capsulatus |
| 8.5 | - |
activated by Mg2+ equimolar to ATP | Rhodobacter capsulatus |
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