Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | activates | Arthrobacter globiformis | |
acetyl-CoA | in absence of acetyl-CoA the maximal rate of oxaloacetate synthesis is 4% of that obtained in presence of saturating concentrations of acetyl-CoA | Arthrobacter globiformis |
General Stability | Organism |
---|---|
acetyl-CoA and sodium pyruvate protects against cold inactivation. L-Asp decreases inactivation, 1 M sucrose gives complete protection | Arthrobacter globiformis |
the presence of KCl at 10, 20, 50, and 100 mM potassium phosphate buffer-KCl buffer at 0°C leads to 85%, 44%, 17% and 0% loss of activity | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Avidin | - |
Arthrobacter globiformis | |
L-Asp | - |
Arthrobacter globiformis | |
Na+ | slight | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | - |
Arthrobacter globiformis | |
0.33 | - |
HCO3- | - |
Arthrobacter globiformis | |
1.86 | - |
pyruvate | - |
Arthrobacter globiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | increases activity by a maximum of 27% at 70 mM | Arthrobacter globiformis | |
Mg2+ | activates | Arthrobacter globiformis | |
Mg2+ | Km: 0.7 mM | Arthrobacter globiformis | |
NH4+ | increases activity by a maximum of 39% at 80 mM | Arthrobacter globiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
4 * 130000, SDS-PAGE | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter globiformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Arthrobacter globiformis |
Storage Stability | Organism |
---|---|
0°C, 10 mM potassium phosphate, 10 mM KCl, 1 mM EDTA, 0.1 mM DTT, pH 6.8, potassium phosphate-KCl buffer, 50% loss of activity after 10 min | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Arthrobacter globiformis | ADP + phosphate + oxaloacetate | - |
? | |
ATP + pyruvate + HCO3- | ATP in form of MgATP2- | Arthrobacter globiformis | ADP + phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 130000, SDS-PAGE | Arthrobacter globiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
cold inactivation | Arthrobacter globiformis |
- |
- |
10 mM potassium phosphate, 10 mM KCl, 1 mM EDTA, 0.1 mM DTT, pH 6.8, potassium phosphate-KCl buffer, 50% loss of activity after 10 min | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
in presence of 5 mM Mn2+ | Arthrobacter globiformis |
7.6 | - |
in presence of 5 mM Mg2+ | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | enzyme contains biotin | Arthrobacter globiformis |