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Literature summary for 6.4.1.1 extracted from
- Isolation and characterization of pyruvate carboxylase from the extremely thermophilic Bacillus caldotenax (1983), Biochem. Soc. Trans., 11, 741-742.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | allosteric activator. Concentrations eliciting maximal activity are 0.013 mM, 0.042 mM and 0.084 mM at assay temperatures of 45°C, 55°C, and 65°C respectively | [Bacillus] caldotenax |  |
General Stability
| General Stability | Organism |
|---|---|
| stable to exposure to 6 M urea for up to 2.5 h at 20°C | [Bacillus] caldotenax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Avidin | strong | [Bacillus] caldotenax | |
| L-Asp | - |
[Bacillus] caldotenax | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | contains 4 atoms of zinc per molecule | [Bacillus] caldotenax | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 136000 | - |
x * 136000 | [Bacillus] caldotenax |
| 537000 | - |
- |
[Bacillus] caldotenax |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | [Bacillus] caldotenax | meets anaplerotic and/or biosynthetic needs in metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Bacillus] caldotenax | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Bacillus] caldotenax |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
- |
[Bacillus] caldotenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | - |
[Bacillus] caldotenax | ADP + phosphate + oxaloacetate | - |
? | |
| ATP + pyruvate + HCO3- | meets anaplerotic and/or biosynthetic needs in metabolism | [Bacillus] caldotenax | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 136000 | [Bacillus] caldotenax |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 7, half-life: 1 h | [Bacillus] caldotenax |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | enzyme contains biotin | [Bacillus] caldotenax | |
| biotin | enzyme contains covalently bound D-biotin | [Bacillus] caldotenax | |
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