Protein Variants | Comment | Organism |
---|---|---|
W170T | increased Km compared to the wild type enzyme | Escherichia coli |
W175T | increased Km compared to the wild type enzyme | Escherichia coli |
W213T | increased Km compared to the wild type enzyme | Escherichia coli |
W437T | increased Km compared to the wild type enzyme | Escherichia coli |
W461T | increased Km compared to the wild type enzyme | Escherichia coli |
W71T | increased Km compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
ATP | wild type enzyme, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli | |
0.51 | - |
ATP | mutant enzyme W437T, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli | |
0.75 | - |
ATP | mutant enzyme W213T, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli | |
1.28 | - |
ATP | mutant enzyme W71T, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli | |
1.34 | - |
ATP | mutant enzyme W461T, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli | |
1.57 | - |
ATP | mutant enzyme W170T, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli | |
1.94 | - |
ATP | mutant enzyme W175T, at 25°C in 50 mM HEPES buffer, pH 7.5 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S-300 gel filtration and Resource-Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Carbamoylphosphate synthetase II | - |
Escherichia coli |
CPS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |