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Literature summary for 6.3.5.5 extracted from

  • Yefimenko, I.; Fresquet, V.; Marco-Marin, C.; Rubio, V.; Cervera, J.
    Understanding carbamoyl phosphate synthetase deficiency: impact of clinical mutations on enzyme functionality (2005), J. Mol. Biol., 349, 127-141.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A126M mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
N301K mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
P360L mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
Q262P mutation causes marked enzyme instability, mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
R169H mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
R675L mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
S789P mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli
V640R mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
ATP pH 8.0, 37°C, wild-type enzyme Escherichia coli
0.32
-
ATP pH 8.0, 25°C, wild-type enzyme Escherichia coli
0.45
-
ATP pH 8.0, 25°C, mutant enzyme Q262P Escherichia coli
0.64
-
HCO3- pH 8.0, 37°C, mutant enzyme P360L Escherichia coli
0.76
-
HCO3- pH 8.0, 25°C, mutant enzyme Q262P Escherichia coli
0.76
-
ATP pH 8.0, 37°C, mutant enzyme R675L Escherichia coli
0.87
-
ATP pH 8.0, 37°C, mutant enzyme A126M Escherichia coli
1.14
-
ATP pH 8.0, 37°C, mutant enzyme R169H Escherichia coli
1.33
-
HCO3- pH 8.0, 25°C, wild-type enzyme Escherichia coli
1.9
-
HCO3- pH 8.0, 37°C, wild-type enzyme Escherichia coli
3.02
-
ATP pH 8.0, 37°C, mutant enzyme V640R Escherichia coli
3.65
-
HCO3- pH 8.0, 37°C, mutant enzyme V640R Escherichia coli
4.46
-
HCO3- pH 8.0, 37°C, mutant enzyme R675L Escherichia coli
8.13
-
ATP pH 8.0, 37°C, mutant enzyme S789P Escherichia coli
8.36
-
ATP pH 8.0, 37°C, mutant enzyme P360L Escherichia coli
12.6
-
HCO3- pH 8.0, 37°C, mutant enzyme R169H Escherichia coli
23.8
-
HCO3- pH 8.0, 37°C, mutant enzyme S789P Escherichia coli
32.5
-
HCO3- pH 8.0, 37°C, mutant enzyme A126M Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
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