Cloned (Comment) | Organism |
---|---|
the plasmids containing the carAB genes are transformed in the RC50 cell line of Escherichia coli for expression of the wild-type and mutant forms of CPS | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E25Q/E383Q | 46.7fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
E25Q/E383Q/E604Q | more than 700fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
E383Q | 1.1fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
E383Q/E916Q | 3.9fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
E577Q | 437.5fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
E604Q | 3.7fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
E916Q | 6.7fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
R306A | more than 700fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
R848A | 233fold decreased turnover number for carbamoyl-phosphate synthesis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.11 | - |
Gln | mutant enzyme E916Q, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.12 | - |
Gln | wild-type enzyme, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.14 | - |
Gln | mutant enzyme E383QE916Q, Gln-dependent ASTP hydrolysis | Escherichia coli | |
0.15 | - |
Gln | mutant enzyme E383Q,Gln-dependent ATP hydrolysis | Escherichia coli | |
0.22 | - |
ATP | mutant enzyme E306A, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.4 | - |
ATP | mutant enzyme R848A, Gln-dependent ATP hydrolysis | Escherichia coli | |
110 | - |
NH4+ | mutant enzyme E383Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
130 | - |
NH4+ | wild-type enzyme, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
160 | - |
NH4+ | mutant enzyme E916Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.097 | - |
NH4+ | mutant enzyme E25Q/E383Q/E604Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
0.1 | - |
Gln | mutant enzyme E306A, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.12 | - |
NH4+ | mutant enzyme E577Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
0.15 | - |
Gln | mutant enzyme E25Q/E383Q/E604Q, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.17 | - |
Gln | mutant enzyme E577Q, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.32 | - |
Gln | mutant enzyme E25Q/E383Q, Gln-dependent ATP hydrolysis | Escherichia coli | |
0.51 | - |
NH4+ | mutant enzyme E25Q/E383Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
0.95 | - |
Gln | mutant enzyme R848A, Gln-dependent ATP hydrolysis | Escherichia coli | |
1.3 | - |
NH4+ | mutant enzyme E604Q, NH4Cl-depebndent ATP hydrolysis | Escherichia coli | |
1.3 | - |
NH4+ | mutant enzyme E916Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
1.5 | - |
Gln | mutant enzyme E604Q, Gln-dependent ATP hydrolysis | Escherichia coli | |
1.7 | - |
NH4+ | mutant enzyme E383Q, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
3.2 | - |
Gln | mutant enzyme E383Q,Gln-dependent ATP hydrolysis | Escherichia coli | |
3.9 | - |
NH4+ | wild-type enzyme, NH4Cl-dependent ATP hydrolysis | Escherichia coli | |
4 | - |
Gln | mutant enzyme E383QE916Q, Gln-dependent ATP hydrolysis | Escherichia coli | |
4 | - |
Gln | wild-type enzyme, Gln-dependent ATP hydrolysis | Escherichia coli | |
4.2 | - |
Gln | mutant enzyme E916Q, Gln-dependent ATP hydrolysis | Escherichia coli |