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Literature summary for 6.3.5.5 extracted from

  • Kim, J.; Raushel, F.M.
    Access to the carbamate tunnel of carbamoyl phosphate synthetase (2004), Arch. Biochem. Biophys., 425, 33-41.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the plasmids containing the carAB genes are transformed in the RC50 cell line of Escherichia coli for expression of the wild-type and mutant forms of CPS Escherichia coli

Protein Variants

Protein Variants Comment Organism
E25Q/E383Q 46.7fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
E25Q/E383Q/E604Q more than 700fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
E383Q 1.1fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
E383Q/E916Q 3.9fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
E577Q 437.5fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
E604Q 3.7fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
E916Q 6.7fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
R306A more than 700fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli
R848A 233fold decreased turnover number for carbamoyl-phosphate synthesis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.11
-
Gln mutant enzyme E916Q, Gln-dependent ATP hydrolysis Escherichia coli
0.12
-
Gln wild-type enzyme, Gln-dependent ATP hydrolysis Escherichia coli
0.14
-
Gln mutant enzyme E383QE916Q, Gln-dependent ASTP hydrolysis Escherichia coli
0.15
-
Gln mutant enzyme E383Q,Gln-dependent ATP hydrolysis Escherichia coli
0.22
-
ATP mutant enzyme E306A, Gln-dependent ATP hydrolysis Escherichia coli
0.4
-
ATP mutant enzyme R848A, Gln-dependent ATP hydrolysis Escherichia coli
110
-
NH4+ mutant enzyme E383Q, NH4Cl-dependent ATP hydrolysis Escherichia coli
130
-
NH4+ wild-type enzyme, NH4Cl-dependent ATP hydrolysis Escherichia coli
160
-
NH4+ mutant enzyme E916Q, NH4Cl-dependent ATP hydrolysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + NH4+ + HCO3-
-
Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.097
-
NH4+ mutant enzyme E25Q/E383Q/E604Q, NH4Cl-dependent ATP hydrolysis Escherichia coli
0.1
-
Gln mutant enzyme E306A, Gln-dependent ATP hydrolysis Escherichia coli
0.12
-
NH4+ mutant enzyme E577Q, NH4Cl-dependent ATP hydrolysis Escherichia coli
0.15
-
Gln mutant enzyme E25Q/E383Q/E604Q, Gln-dependent ATP hydrolysis Escherichia coli
0.17
-
Gln mutant enzyme E577Q, Gln-dependent ATP hydrolysis Escherichia coli
0.32
-
Gln mutant enzyme E25Q/E383Q, Gln-dependent ATP hydrolysis Escherichia coli
0.51
-
NH4+ mutant enzyme E25Q/E383Q, NH4Cl-dependent ATP hydrolysis Escherichia coli
0.95
-
Gln mutant enzyme R848A, Gln-dependent ATP hydrolysis Escherichia coli
1.3
-
NH4+ mutant enzyme E604Q, NH4Cl-depebndent ATP hydrolysis Escherichia coli
1.3
-
NH4+ mutant enzyme E916Q, NH4Cl-dependent ATP hydrolysis Escherichia coli
1.5
-
Gln mutant enzyme E604Q, Gln-dependent ATP hydrolysis Escherichia coli
1.7
-
NH4+ mutant enzyme E383Q, NH4Cl-dependent ATP hydrolysis Escherichia coli
3.2
-
Gln mutant enzyme E383Q,Gln-dependent ATP hydrolysis Escherichia coli
3.9
-
NH4+ wild-type enzyme, NH4Cl-dependent ATP hydrolysis Escherichia coli
4
-
Gln mutant enzyme E383QE916Q, Gln-dependent ATP hydrolysis Escherichia coli
4
-
Gln wild-type enzyme, Gln-dependent ATP hydrolysis Escherichia coli
4.2
-
Gln mutant enzyme E916Q, Gln-dependent ATP hydrolysis Escherichia coli