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Literature summary for 6.3.5.5 extracted from

  • Ahuja, A.; Purcarea, C.; Guy, H.I.; Evans, D.R.
    A novel carbamoyl-phosphate synthetase from Aquifex aeolicus (2001), J. Biol. Chem., 276, 45694-45703.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ornithine
-
Aquifex aeolicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

Inhibitors

Inhibitors Comment Organism Structure
UMP
-
Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE Aquifex aeolicus
63000
-
CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE Aquifex aeolicus
171000
-
-
Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + L-Gln + HCO3- Aquifex aeolicus the product carbamoyl phosphate is the initial intermediate in the biosynthesis of both pyrimidine and arginine 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-
Bacillus subtilis
-
-
-
Cricetinae
-
-
-
Escherichia coli
-
-
-
Methanocaldococcus jannaschii
-
-
-
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aquifex aeolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.041
-
recombinant enzyme Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Bacillus subtilis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3-
-
Pseudomonas aeruginosa 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3-
-
Methanocaldococcus jannaschii 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3-
-
Cricetinae 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3-
-
Aquifex aeolicus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is the initial intermediate in the biosynthesis of both pyrimidine and arginine Aquifex aeolicus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + NH4+ + HCO3-
-
Aquifex aeolicus 2 ADP + phosphate + carbamoyl phosphate
-
?

Subunits

Subunits Comment Organism
heterotrimer CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80 CPS.A-CPS.B complex Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 80 The activity of the CPS.A-CPS.B complex remains unchanged between 25-60 °C and then increases 54% at 70°C. The loss of catalytic activity above 80°C is indicated of thermal denaturation. The large increase in thermal stability at temperatures prior to thermal denaturation is not observed in the CPS.A-CPS.B-glutaminase-subunit complex. Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Aquifex aeolicus