Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ornithine | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H975L | mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine | Escherichia coli |
H975L/N987V | mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine | Escherichia coli |
L421E | mutation prevents tetramer but not dimer formation | Escherichia coli |
L421E/H975L/N987V | mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine | Escherichia coli |
L990A | mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine | Escherichia coli |
N987V | mutation decreases the oligomerisation | Escherichia coli |
N992A | mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
constant specific activity of wild-type enzyme irrespective of the enzyme concentration or of the elution position of the enzyme in the gel filtration system under conditions at which different oligomeric forms predominate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | at low concentrations in presence of UMP | Escherichia coli |
More | in absence of effectors the enzyme behaves as a dissociating system possibly reflecting the dimer-tetramer equilibrium | Escherichia coli |
tetramer | at high concentrations in presence of ornithine | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
UMP | pH 8.0, 24°C | Escherichia coli |