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Literature summary for 6.3.5.5 extracted from

  • Huang, X.; Raushel, F.M.
    An engineered blockage within the ammonia tunnel of carbamoyl phosphate synthetase prevents the use of glutamine as a substrate but not ammonia (2000), Biochemistry, 39, 3240-3247.
    View publication on PubMed
Search for more literature for 6.3.5.5

Protein Variants

Protein Variants Comment Organism
A309C kinetic properties are similar to those of the wild-type enzyme Escherichia coli
A309C/S35C kinetic properties are similar to those of the wild-type enzyme Escherichia coli
A309S kinetic properties are similar to those of the wild-type enzyme Escherichia coli
G293A kinetic properties are similar to those of the wild-type enzyme Escherichia coli
G293I kinetic properties are similar to those of the wild-type enzyme Escherichia coli
G293S kinetic properties are similar to those of the wild-type enzyme Escherichia coli
G359F glutaminase and bicarbonate-dependent ATPase reaction are uncoupled from one another, the mutant enzyme is fully functional when external ammonia is utilized as the nitrogen source but is unable to use glutamine for the synthesis of carbamoyl phosphate Escherichia coli
G359Y glutaminase and bicarbonate-dependent ATPase reaction are uncoupled from one another, the mutant enzyme is fully functional when external ammonia is utilized as the nitrogen source but is unable to use glutamine for the synthesis of carbamoyl phosphate Escherichia coli
S35C kinetic properties are similar to those of the wild-type enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 1 * 42000 + 1 * 118000 Escherichia coli
118000
-
 1 * 42000 + 1 * 118000 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?
additional information existence of an alternate route to the bicarbonate phosphorylation site when ammonia is provided as an external nitrogen source Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 42000 + 1 * 118000 Escherichia coli