Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IMP | allosteric effector | Escherichia coli | |
L-ornithine | positive allosteric activation, E783, T1042, and T1043 are primarily responsible for the binding of ornithine to enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E761A | the residue is a key in the allosteric signal transduction pathway from ornithine to ATP | Escherichia coli |
E783A | the allosteric activation of enzyme by ornithine is completely suppressed | Escherichia coli |
E783K | the allosteric activation of enzyme by ornithine is completely suppressed | Escherichia coli |
E892A | the allosteric activation of enzyme by ornithine is completely suppressed | Escherichia coli |
E892K | the allosteric activation of enzyme by ornithine is completely suppressed | Escherichia coli |
additional information | several mutations increase, reduce, suppresse, or reverse the allosteric effects of ornithine, UMP and IMP, the mutational analysis of ornithine, and potassium binding site strongly supports the proposal for a coupling between the allosteric transduction signal pathways for UMP,IMP and ornithine | Escherichia coli |
T1042K | the residue is responsible for the binding of ornithine to enzyme | Escherichia coli |
T1043K | the allosteric activation of enzyme by ornithine is completely suppressed | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of kcat in absence and presence of L-ornithine, UMP and IMP for wild-type and mutant enzymes | Escherichia coli |