Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribosyl 1-diphosphate | stimulates | Rattus norvegicus |
General Stability | Organism |
---|---|
enzyme is most stable in the presence of 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol. MgATP2-, MgUTP2-, and 5-phospho-alpha-D-ribosyl 1-diphosphate effectively stabilize | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | - |
Rattus norvegicus | |
ADP | competitive with MgATP2- | Rattus norvegicus | |
Ala | inhibits glutamine hydrolysis and Gln-dependent carbamoyl phosphate synthesis | Rattus norvegicus | |
CTP | - |
Rattus norvegicus | |
dUDP | - |
Rattus norvegicus | |
dUTP | - |
Rattus norvegicus | |
Gly | - |
Rattus norvegicus | |
Mersalyl | - |
Rattus norvegicus | |
polyamines | e.g. spermine, spermidine or putrescine | Rattus norvegicus | |
putrescine | - |
Rattus norvegicus | |
spermidine | - |
Rattus norvegicus | |
spermine | - |
Rattus norvegicus | |
UDP | - |
Rattus norvegicus | |
UDPglucose | - |
Rattus norvegicus | |
UTP | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
L-Gln | in 10% glycerol, pH 7 | Rattus norvegicus | |
1 | - |
HCO3- | in 30% dimethyl sulfoxide | Rattus norvegicus | |
1.7 | - |
ATP | MgATP2-, in 10% glycerol, pH 7 | Rattus norvegicus | |
10 | - |
HCO3- | in 10% glycerol, pH 7 | Rattus norvegicus | |
15 | - |
NH4+ | in 10% glycerol, pH 7 | Rattus norvegicus | |
20 | - |
HCO3- | in absence of cryoprotectants | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for maximal activity | Rattus norvegicus | |
K+ | Km: 18 mM | Rattus norvegicus | |
Mg2+ | optimal concentration: 5 mM | Rattus norvegicus | |
Mg2+ | free Mg2+ is required in addition to MgATP2- | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase has a MW of 870000 as determined by sedimentation equilibrium studies and 210000 by SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma ascites cell | AH13 | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-80°C, 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol, stable for 6 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
ATP + L-glutamine + hydrogencarbonate + H2O | ATP in form of MgATP2- | Rattus norvegicus | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.2 | Gln-dependent activity | Rattus norvegicus |
7.8 | - |
NH4+-dependent activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
half-life: 20 min | Rattus norvegicus |