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Literature summary for 6.3.5.5 extracted from

  • Mori, M.; Tatibana, M.
    A multienzyme complex of carbamoyl-phosphate synthase (glutamine):aspartate carbamoyltransferase:dihydroorotase (rat ascites hepatoma cells and rat liver) (1978), Methods Enzymol., 51, 111-121.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5-phospho-alpha-D-ribosyl 1-diphosphate stimulates Rattus norvegicus

General Stability

General Stability Organism
enzyme is most stable in the presence of 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol. MgATP2-, MgUTP2-, and 5-phospho-alpha-D-ribosyl 1-diphosphate effectively stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate
-
Rattus norvegicus
ADP competitive with MgATP2- Rattus norvegicus
Ala inhibits glutamine hydrolysis and Gln-dependent carbamoyl phosphate synthesis Rattus norvegicus
CTP
-
Rattus norvegicus
dUDP
-
Rattus norvegicus
dUTP
-
Rattus norvegicus
Gly
-
Rattus norvegicus
Mersalyl
-
Rattus norvegicus
polyamines e.g. spermine, spermidine or putrescine Rattus norvegicus
putrescine
-
Rattus norvegicus
spermidine
-
Rattus norvegicus
spermine
-
Rattus norvegicus
UDP
-
Rattus norvegicus
UDPglucose
-
Rattus norvegicus
UTP
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
L-Gln in 10% glycerol, pH 7 Rattus norvegicus
1
-
HCO3- in 30% dimethyl sulfoxide Rattus norvegicus
1.7
-
ATP MgATP2-, in 10% glycerol, pH 7 Rattus norvegicus
10
-
HCO3- in 10% glycerol, pH 7 Rattus norvegicus
15
-
NH4+ in 10% glycerol, pH 7 Rattus norvegicus
20
-
HCO3- in absence of cryoprotectants Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for maximal activity Rattus norvegicus
K+ Km: 18 mM Rattus norvegicus
Mg2+ optimal concentration: 5 mM Rattus norvegicus
Mg2+ free Mg2+ is required in addition to MgATP2- Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase has a MW of 870000 as determined by sedimentation equilibrium studies and 210000 by SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma ascites cell AH13 Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol, stable for 6 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Rattus norvegicus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + NH4+ + HCO3-
-
Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
?
ATP + L-glutamine + hydrogencarbonate + H2O ATP in form of MgATP2- Rattus norvegicus ADP + phosphate + L-glutamate + carbamoyl phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2 Gln-dependent activity Rattus norvegicus
7.8
-
NH4+-dependent activity Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
half-life: 20 min Rattus norvegicus