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Literature summary for 6.3.5.5 extracted from

  • Aoki, T.; Oya, H.
    Kinetic properties of carbamoyl-phosphate synthetase II (glutamine-hydrolyzing) in the parasitic protozoan Crithidia fasciculata and separation of the enzyme from aspartate carbamoyltransferase (1987), Comp. Biochem. Physiol. B, 87, 143-150.
    View publication on PubMed
Search for more literature for 6.3.5.5

Activating Compound

Activating Compound Comment Organism Structure
N-acetylglutamate no requirement for N-acetyl-L-glutamate Crithidia fasciculata

Inhibitors

Inhibitors Comment Organism Structure
HCO3- 20 mM Crithidia fasciculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 L-Gln at 8.6% dimethyl sulfoxide plus 1.4% glycerol Crithidia fasciculata
0.14
-
 L-Gln at 2.0% dimethyl sulfoxide plus 8.0% glycerol Crithidia fasciculata
0.27
-
 L-Gln at 2.0% dimethyl sulfoxide plus 0.3% glycerol Crithidia fasciculata
1.2
-
 HCO3- HCO3-, at 8.6% dimethyl sulfoxide plus 1.4% glycerol Crithidia fasciculata
1.7
-
 ATP HCO3-, at 2.0% dimethyl sulfoxide plus 0.3% glycerol or 8.0% glycerol Crithidia fasciculata
1.7
-
 ATP at 2.0% dimethyl sulfoxide plus 0.3% glycerol or 8.0% glycerol Crithidia fasciculata
26
-
 NH4+ at 2.0% dimethyl sulfoxide plus 0.3% glycerol Crithidia fasciculata

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00095
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
 Crithidia fasciculata 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Crithidia fasciculata 2 ADP + phosphate + carbamoyl phosphate
-
 ?
ATP + L-glutamine + hydrogencarbonate + H2O ATP in form of MgATP2- Crithidia fasciculata ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 15 min, stable in absence of dimetyl sulfoxide and glycerol Crithidia fasciculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 highest activity in the pH-range 6.7-8.8 Crithidia fasciculata

pH Range

pH Minimum pH Maximum Comment Organism
6.7 8.5 6.7: highest activity in the pH-range 6.7-8.5, pH 7.2: 95% of the activity at pH 6.7, pH 8.5: 64% of the activity at pH 6.7 Crithidia fasciculata