Cloned (Comment) | Organism |
---|---|
PVAS2 is cloned into the expression vector pGEXKG and overexpressed as a glutathione S-transferase fusion protein. PVAS2 is cloned into pUC18, expression in Escherichia coli asparagine-auxotroph ER4813 | Phaseolus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | Phaseolus vulgaris | the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | of mature plants | Phaseolus vulgaris | - |
root nodule | detection in early stages of nodule development, no detection in nodules that have become competent for nitrogen fixation | Phaseolus vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | - |
Phaseolus vulgaris | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + L-glutamine | the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris | Phaseolus vulgaris | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asparagine synthetase | - |
Phaseolus vulgaris |
PVAS2 | - |
Phaseolus vulgaris |
type -II asparagine synthetase | - |
Phaseolus vulgaris |