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Literature summary for 6.3.5.4 extracted from

  • Galvez-Valdivieso, G.; Osuna, D.; Maldonado, J.M.; Pineda, M.; Aguilar, M.
    Purification of a functional asparagine synthetase (PVAS2) from common bean (Phaseolus vulgaris), a protein predominantly found in root tissues (2004), Plant Sci., 168, 89-94.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
PVAS2 is cloned into the expression vector pGEXKG and overexpressed as a glutathione S-transferase fusion protein. PVAS2 is cloned into pUC18, expression in Escherichia coli asparagine-auxotroph ER4813 Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + L-glutamine Phaseolus vulgaris the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris AMP + diphosphate + L-asparagine + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
root of mature plants Phaseolus vulgaris
-
root nodule detection in early stages of nodule development, no detection in nodules that have become competent for nitrogen fixation Phaseolus vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + L-glutamine
-
Phaseolus vulgaris AMP + diphosphate + L-asparagine + L-glutamate
-
?
ATP + L-aspartate + L-glutamine the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris Phaseolus vulgaris AMP + diphosphate + L-asparagine + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
Asparagine synthetase
-
Phaseolus vulgaris
PVAS2
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Phaseolus vulgaris
type -II asparagine synthetase
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Phaseolus vulgaris