Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.4 extracted from

  • Nakano, K.; Suzuki, T.; Hayakawa, T.; Yamaya, T.
    Organ and cellular localization of asparagine synthetase in rice plants (2000), Plant Cell Physiol., 41, 874-880.
    View publication on PubMed
Search for more literature for 6.3.5.4

Cloned(Commentary)

Cloned (Comment) Organism
a fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid, expression in Escherichia coli Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oryza sativa nitrogen metabolism, asparagine synthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cv. Sasanishiki
-

Purification (Commentary)

Purification (Comment) Organism
recombinant fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
caryopsis high content of AS in grains in the middle stage of ripening, in vascular tissues Oryza sativa
-
leaf high content of AS in leaf sheath at the second position from the fully expanded top leaf, the contents gradually decreases in leaf sheaths as a function of increasing age, in vascular tissues Oryza sativa
-
additional information organ and cellular localization Oryza sativa
-
spikelet during the ripening of the spikelets AS contents increases during the first 21 days after flowering, then declines rapidly Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + L-glutamine
-
 Oryza sativa AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
additional information nitrogen metabolism, asparagine synthesis Oryza sativa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Oryza sativa