Literature summary for 6.3.5.4 extracted from

Boehlein, S.K.; Schuster, S.M.; Richards, N.G.J.
Glutamic acid gamma-monohydroxylamine are alternate substrates for Escherichia coli asparagine synthetase B (1996), Biochemistry, 35, 3031-3037.

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Protein Variants

Protein Variants	Comment	Organism
N74A	mutant N74A, in which Asn is replaced by Ala can also use NH2OH as an alternative substrate to NH4+ and catalyze the hydrolysis of L-glutamic acid gamma-monohydroxamate	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	L-Gln	mutant N74A	Escherichia coli
0.09	-	L-glutamic acid gamma-monohydroxamate	mutant N74A	Escherichia coli
0.26	-	L-glutamic acid gamma-monohydroxamate	wild-type enzyme	Escherichia coli
0.69	-	L-Gln	wild-type enzyme	Escherichia coli
5.24	-	Gln	mutant C99A	Escherichia coli
11.5	-	hydroxylamine	wild-type enzyme	Escherichia coli
13	-	NH4+	mutant N74A	Escherichia coli
15.7	-	NH4+	wild-type enzyme	Escherichia coli
17.1	-	hydroxylamine	mutant N74A	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	wild-type and mutant N74A	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-Asp + L-Gln	-	Escherichia coli	AMP + diphosphate + Asn + Glu	-	?
ATP + L-Asp + NH2OH	-	Escherichia coli	AMP + diphosphate + beta-aspartylhydroxamate	-	?
L-Glutamic acid gamma-monohydroxamate + H2O	-	Escherichia coli	Hydroxylamine + Glu	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.05	-	L-Gln	mutant N74A	Escherichia coli
0.1	-	L-glutamic acid gamma-monohydroxamate	mutant N74A	Escherichia coli
0.15	-	L-glutamic acid gamma-monohydroxamate	wild-type	Escherichia coli
0.59	-	NH4+	wild-type	Escherichia coli
0.65	-	NH4+	mutant N74A	Escherichia coli
1.01	-	L-Gln	wild-type enzyme	Escherichia coli
1.03	-	hydroxylamine	wild-type enzyme	Escherichia coli
1.17	-	hydroxylamine	N74A mutant	Escherichia coli

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Release 2023.1 (January 2023)