Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.4 extracted from

  • Rognes, S.E.
    Anion regulation of lupin Asparagine+ synthetase: chloride activation of the glutamine-utilizing reactions (1980), Phytochemistry, 19, 2287-2293.
No PubMed abstract available
Search for more literature for 6.3.5.4

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 20 mM, 12-15% inhibition Lupinus luteus
oxaloacetate 20 mM, 40% inhibition Lupinus luteus
pyruvate 20 mM, 12-15% inhibition Lupinus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
Br- strong activation of Asn synthesis and Gln hydrolysis. The synthetase reactions with NH3 or NH2OH are only slightly stimulated Lupinus luteus
Cl- strong activation of Asn synthesis and Gln hydrolysis, competitive activator with respect to Gln and a noncompetitive activator with respect to MgATP2- and Asp. Cl- changes the substrate saturation kinetics of Gln from negatively cooperative to normal hyperbolic and causes a 50fold increase in the affinity for Gln. Inherent glutaminase activity is enhanced 30fold. The synthetase reactions with NH4+ or NH2OH are only slightly stimulated Lupinus luteus
CN- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus
F- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus
I- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus
NO3- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon of seedlings Lupinus luteus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + L-Gln
-
 Lupinus luteus AMP + diphosphate + Asn + Glu
-
 ?
ATP + L-Asp + NH2OH
-
 Lupinus luteus AMP + diphosphate + beta-aspartylhydroxamate
-
 ?
ATP + L-Asp + NH3
-
 Lupinus luteus AMP + diphosphate + Asn
-
 ?
additional information enzyme has inherent glutaminase activity Lupinus luteus ?
-
 ?