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Literature summary for 6.3.5.2 extracted from

  • Franco, T.M.; Rostirolla, D.C.; Ducati, R.G.; Lorenzini, D.M.; Basso, L.A.; Santos, D.S.
    Biochemical characterization of recombinant guaA-encoded guanosine monophosphate synthetase (EC 6.3.5.2) from Mycobacterium tuberculosis H37Rv strain (2012), Arch. Biochem. Biophys., 517, 1-11.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
ATP substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
ATP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis
1.24
-
L-glutamine at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
2 * 56000, SDS-PAGE Mycobacterium tuberculosis
137000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + XMP + L-glutamine + H2O Mycobacterium tuberculosis
-
AMP + diphosphate + GMP + L-glutamate
-
?
ATP + XMP + L-glutamine + H2O Mycobacterium tuberculosis H37Rv
-
AMP + diphosphate + GMP + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, butyl Sepharose column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-80°C, in 50 mM Tris pH 7.5, at least 3 months, no loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + XMP + L-glutamine + H2O
-
Mycobacterium tuberculosis AMP + diphosphate + GMP + L-glutamate
-
?
ATP + XMP + L-glutamine + H2O
-
Mycobacterium tuberculosis H37Rv AMP + diphosphate + GMP + L-glutamate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 56000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
GMP synthetase
-
Mycobacterium tuberculosis
GMPS
-
Mycobacterium tuberculosis
GUA
-
Mycobacterium tuberculosis
guanosine monophosphate synthetase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
XMP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis
2.39
-
ATP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis
3.05
-
L-glutamine at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
21
-
ATP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis