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Literature summary for 6.3.5.2 extracted from
- GMP synthetase (Escherichia coli) (1978), Methods Enzymol., 51, 213-218.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-Amino-9-D-psicofuranosylpurine | i.e. psicofuranine, irreversible inhibition, dependent on presence of XMP and diphosphate | Escherichia coli |  |
| 6-diazo-5-oxo-L-norleucine | - |
Escherichia coli | |
| guanidine hydrochloride | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
XMP | - |
Escherichia coli | |
| 0.53 | - |
ATP | - |
Escherichia coli | |
| 1 | - |
NH4+ | Gln | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
2 * 63000, SDS-PAGE, sedimentation equilibrium measurement in 6.0 M guanidine hydrochloride | Escherichia coli |
| 126000 | - |
sedimentation equilibrium measurement | Escherichia coli |
| 128000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B96 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.09 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + XMP + Gln | identical Km value for NH4+ and Gln | Escherichia coli | AMP + diphosphate + GMP + Glu | - |
? | |
| ATP + XMP + Gln | identical Km value for NH4+ and Gln | Escherichia coli B96 | AMP + diphosphate + GMP + Glu | - |
? | |
| ATP + XMP + NH4+ | identical Km value for NH4+ and Gln | Escherichia coli | AMP + diphosphate + GMP | - |
? | |
| ATP + XMP + NH4+ | identical Km value for NH4+ and Gln | Escherichia coli B96 | AMP + diphosphate + GMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 63000, SDS-PAGE, sedimentation equilibrium measurement in 6.0 M guanidine hydrochloride | Escherichia coli |
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