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Literature summary for 6.3.4.9 extracted from
- Purification and properties of the synthetase catalyzing the biotination of the aposubunit of transcarboxylase from Propionibacterium shermanii (1988), FASEB J., 2, 2396-2401.
General Stability
| General Stability | Organism |
|---|---|
| divalent metal is required for storage stability, Mg2+, Mn2+, Co2+, Zn2+, or Ni2+ | Propionibacterium freudenreichii subsp. shermanii |
| loses activity by repeated freezing and thawing | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, inhibition at high concentrations | Propionibacterium freudenreichii subsp. shermanii |  |
| Cu2+ | - |
Propionibacterium freudenreichii subsp. shermanii | |
| EDTA | - |
Propionibacterium freudenreichii subsp. shermanii | |
| Hg2+ | - |
Propionibacterium freudenreichii subsp. shermanii | |
| Zn2+ | activates, inhibition at high concentrations | Propionibacterium freudenreichii subsp. shermanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
1.3 S subunit of apotranscarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii | |
| 0.038 | - |
ATP | - |
Propionibacterium freudenreichii subsp. shermanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+, Km: 0.0017 mM | Propionibacterium freudenreichii subsp. shermanii | |
| Mg2+ | divalent metal ion required | Propionibacterium freudenreichii subsp. shermanii | |
| Mg2+ | affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Mg2+ | Km: 0.0019 mM | Propionibacterium freudenreichii subsp. shermanii | |
| Mn2+ | affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Mn2+ | Km: 0.0015 mM | Propionibacterium freudenreichii subsp. shermanii | |
| Ni2+ | affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+, Km: 0.0013 mM | Propionibacterium freudenreichii subsp. shermanii | |
| Zn2+ | affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+, Km: 0.0003 mM | Propionibacterium freudenreichii subsp. shermanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0124 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 6 months | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + 1.3 S subunit of apotranscarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
| ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase] | - |
Propionibacterium freudenreichii subsp. shermanii | AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | about 30% of maximal activity at 20°C and 60°C | Propionibacterium freudenreichii subsp. shermanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
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