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Literature summary for 6.3.4.4 extracted from

  • Gorrell, A.; Wang, W.; Underbakke, E.; Hou, Z.; Honzatko, R.B.; Fromm, H.J.
    Determinants of L-L-aspartate and IMP recognition in Escherichia coli adenylosuccinate synthetase (2002), J. Biol. Chem., 277, 8817-8821.
    View publication on PubMed
Search for more literature for 6.3.4.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
T128A site-directed mutagenesis Escherichia coli
T129A site-directed mutagenesis Escherichia coli
T300A site-directed mutagenesis Escherichia coli
T301A site-directed mutagenesis Escherichia coli
V273A site-directed mutagenesis Escherichia coli
V273N site-directed mutagenesis Escherichia coli
V273T site-directed mutagenesis Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
fumarate
-
 Escherichia coli
Maleate
-
 Escherichia coli
succinate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 IMP pH 7.7, 25°C, mutant V273A Escherichia coli
0.019
-
 IMP pH 7.7, 25°C, mutant T300A Escherichia coli
0.021
-
 GTP pH 7.7, 25°C, mutant V273N Escherichia coli
0.024
-
 IMP pH 7.7, 25°C, mutant V273N Escherichia coli
0.026
-
 GTP pH 7.7, 25°C, wild-type Escherichia coli
0.028
-
 IMP pH 7.7, 25°C, wild-type Escherichia coli
0.031
-
 GTP pH 7.7, 25°C, mutant V273A Escherichia coli
0.033
-
 IMP pH 7.7, 25°C, mutant T300V Escherichia coli
0.043
-
 IMP pH 7.7, 25°C, mutant V273T Escherichia coli
0.045
-
 GTP pH 7.7, 25°C, mutant V273T Escherichia coli
0.054
-
 GTP pH 7.7, 25°C, mutant T128A Escherichia coli
0.056
-
 GTP pH 7.7, 25°C, mutant T300V Escherichia coli
0.057
-
 GTP pH 7.7, 25°C, mutant T301A, hydroxylamine as substrate Escherichia coli
0.066
-
 IMP pH 7.7, 25°C, wild-type, hydroxylamine as substrate Escherichia coli
0.074
-
 GTP pH 7.7, 25°C, mutant V273T, hydroxylamine as substrate Escherichia coli
0.077
-
 IMP pH 7.7, 25°C, mutant V273T, hydroxylamine as substrate Escherichia coli
0.113
-
 IMP pH 7.7, 25°C, mutant V273N, hydroxylamine as substrate Escherichia coli
0.14
-
 GTP pH 7.7, 25°C, mutant V273A, hydroxylamine as substrate Escherichia coli
0.14
-
 IMP pH 7.7, 25°C, mutant V273A, hydroxylamine as substrate Escherichia coli
0.17
-
 L-aspartate pH 7.7, 25°C, mutant T128A Escherichia coli
0.17
-
 GTP pH 7.7, 25°C, mutant T300A Escherichia coli
0.18
-
 GTP pH 7.7, 25°C, mutant V273N, hydroxylamine as substrate Escherichia coli
0.19
-
 GTP pH 7.7, 25°C, wild-type, hydroxylamine as substrate Escherichia coli
0.23
-
 L-aspartate pH 7.7, 25°C, wild-type Escherichia coli
0.24
-
 L-aspartate pH 7.7, 25°C, mutant T129A Escherichia coli
0.31
-
 IMP pH 7.7, 25°C, mutant T128A Escherichia coli
0.32
-
 IMP pH 7.7, 25°C, mutant T301A, hydroxylamine as substrate Escherichia coli
0.82
-
 L-aspartate pH 7.7, 25°C, mutant T300V Escherichia coli
0.89
-
 IMP pH 7.7, 25°C, mutant T129A Escherichia coli
1.7
-
 GTP pH 7.7, 25°C, mutant T129A Escherichia coli
3.4
-
 L-aspartate pH 7.7, 25°C, mutant V273T Escherichia coli
6.2
-
 L-aspartate pH 7.7, 25°C, mutant T300A Escherichia coli
7
-
 L-aspartate pH 7.7, 25°C, mutant V273A Escherichia coli
9
-
 L-aspartate pH 7.7, 25°C, mutant V273N Escherichia coli
91
-
 hydroxylamine pH 7.7, 25°C, mutant T301A Escherichia coli
110
-
 hydroxylamine pH 7.7, 25°C, mutant V273N Escherichia coli
140
-
 hydroxylamine pH 7.7, 25°C, mutant V273T Escherichia coli
230
-
 hydroxylamine pH 7.7, 25°C, wild-type Escherichia coli
255
-
 hydroxylamine pH 7.7, 25°C, mutant V273A Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + IMP + L-aspartate Escherichia coli governs the first committed step in de novo biosynthesis of AMP plays a significant role in de novo purine nucleotide biosynthesis, the purine nucleotide cycle, and/or salvage pathway for nucleotides GDP + phosphate + adenylosuccinate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7D4
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + hydroxylamine
-
 Escherichia coli GDP + phosphate + ?
-
 ?
GTP + IMP + L-aspartate
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
GTP + IMP + L-aspartate governs the first committed step in de novo biosynthesis of AMP plays a significant role in de novo purine nucleotide biosynthesis, the purine nucleotide cycle, and/or salvage pathway for nucleotides Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
Adenylosuccinate synthetase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 GTP pH 7.7, 25°C, mutant V273N Escherichia coli
0.08
-
 hydroxylamine pH 7.7, 25°C, mutant V273T Escherichia coli
0.21
-
 hydroxylamine pH 7.7, 25°C, mutant V273A Escherichia coli
0.24
-
 hydroxylamine pH 7.7, 25°C, mutant T301A Escherichia coli
0.29
-
 hydroxylamine pH 7.7, 25°C, mutant V273N Escherichia coli
0.42
-
 GTP pH 7.7, 25°C, mutant T129A Escherichia coli
0.46
-
 GTP pH 7.7, 25°C, mutant T128A Escherichia coli
0.5
-
 hydroxylamine pH 7.7, 25°C, wild-type Escherichia coli
0.64
-
 GTP pH 7.7, 25°C, mutant T300A Escherichia coli
1
-
 GTP pH 7.7, 25°C, mutant V273A Escherichia coli
1
-
 GTP pH 7.7, 25°C, wild-type Escherichia coli
1.99
-
 GTP pH 7.7, 25°C, mutant V273T Escherichia coli
2.94
-
 GTP pH 7.7, 25°C, mutant V273T Escherichia coli
3.3
-
 GTP pH 7.7, 25°C, mutant T300V Escherichia coli
6.08
-
 GTP pH 7.7, 25°C, mutant T300A Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
 fumarate pH 7.7, 25°C, mutant V273N Escherichia coli
0.078
-
 Maleate pH 7.7, 25°C, mutant V273T Escherichia coli
0.08
-
 succinate pH 7.7, 25°C, mutant V273T Escherichia coli
0.18
-
 fumarate pH 7.7, 25°C, mutant V273A Escherichia coli
0.21
-
 Maleate pH 7.7, 25°C, mutant V273N Escherichia coli
0.64
-
 fumarate pH 7.7, 25°C, mutant V273T Escherichia coli
0.89
-
 succinate pH 7.7, 25°C, wild-type Escherichia coli
1
-
 Maleate pH 7.7, 25°C, mutant V273A Escherichia coli
3.1
-
 Maleate pH 7.7, 25°C, wild-type Escherichia coli