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Literature summary for 6.3.4.4 extracted from

  • Lipps, G.; Krauss, G.
    Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein (1999), Biochem. J., 341, 537-543.
No PubMed abstract available

Application

Application Comment Organism
agriculture target of herbicides Saccharomyces cerevisiae
medicine target of drugs Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed Saccharomyces cerevisiae

General Stability

General Stability Organism
purified enzyme is not stable, loses its activity within weeks Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
adenylosuccinate
-
Saccharomyces cerevisiae
AMP
-
Saccharomyces cerevisiae
ATP
-
Saccharomyces cerevisiae
Cl- weak inhibitor Saccharomyces cerevisiae
GMP potent inhibitor, competitive inhibition towards IMP Saccharomyces cerevisiae
additional information negligible inhibition by adenine nucleotides Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
GTP pH 8.0, 25°C, native enzyme Saccharomyces cerevisiae
0.081
-
GTP pH 8.0, 25°C, recombinant enzyme Saccharomyces cerevisiae
0.2
-
IMP pH 8.0, 25°C, recombinant enzyme Saccharomyces cerevisiae
0.3
-
IMP pH 8.0, 25°C, native enzyme Saccharomyces cerevisiae
1
-
L-aspartate pH 8.0, 25°C, native enzyme Saccharomyces cerevisiae
1.65
-
L-aspartate pH 8.0, 25°C, recombinant enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
SDS-PAGE Saccharomyces cerevisiae
48000
-
theoretical molecular mass Saccharomyces cerevisiae
77000
-
gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + IMP + L-aspartate Saccharomyces cerevisiae key enzyme for the synthesis of adenosine de novo, first committed step in synythesis of adenosine, located at the branchpoint of AMP and GMP synthesis GDP + phosphate + adenylosuccinate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, remains stable for months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-aspartate
-
Saccharomyces cerevisiae GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate key enzyme for the synthesis of adenosine de novo, first committed step in synythesis of adenosine, located at the branchpoint of AMP and GMP synthesis Saccharomyces cerevisiae GDP + phosphate + adenylosuccinate
-
?
additional information ATP is no substrate Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
adenosylsuccinate synthase
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.057
-
GMP pH 8.0, 25°C Saccharomyces cerevisiae
2.7
-
adenylosuccinate pH 8.0, 25°C Saccharomyces cerevisiae
4
-
ATP pH 8.0, 25°C Saccharomyces cerevisiae
6
-
AMP pH 8.0, 25°C Saccharomyces cerevisiae
8
-
Cl- pH 8.0, 25°C Saccharomyces cerevisiae