Protein Variants | Comment | Organism |
---|---|---|
R143L | mutant enzyme R143L with no change in catalytic constant or Km for Asp, but significantly impaired nucleotide binding, 60fold increased Km for IMP and 10fold increased Km for GTP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanosine 5'-O-[S-(4-bromo-2,3-dioxobutyl)thio]phosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
GTP | wild-type | Escherichia coli | |
0.03 | - |
Asp | IMP, wild-type | Escherichia coli | |
0.23 | - |
Asp | Asp, wild-type | Escherichia coli | |
0.269 | - |
GTP | mutant R143L | Escherichia coli | |
0.34 | - |
allopurinol ribonucleotide | Asp, mutant R143L | Escherichia coli | |
1.73 | - |
IMP | mutant R143L | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant R143L | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 4-hydroxypyrazolo[3,4-d]pyrimidine ribonucleotide + L-Asp | - |
Escherichia coli | GDP + phosphate + 4-aminopyrazolo[3,4-d]pyrimidine ribonucleotide | - |
? | |
GTP + IMP + L-Asp | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |