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Literature summary for 6.3.2.4 extracted from

  • Sato, M.; Kirimura, K.; Kino, K.
    Substrate specificity of thermostable D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589 (2006), Biosci. Biotechnol. Biochem., 70, 2790-2792.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
ATCC 43589
-
Thermotoga maritima P46805 strain ATCC 43589
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.071
-
substrate beta-alanine, 60°C Thermotoga maritima
0.12
-
substrate D-threonine, 60°C Thermotoga maritima
0.2
-
substrate glycine, 60°C Thermotoga maritima
0.88
-
substrate D-serine, 60°C Thermotoga maritima
1.6
-
substrate D-cysteine, 60°C Thermotoga maritima
5.5
-
substrate D-alanine, 60°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-Ala at 60°C activity is 1.3% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + ?
-
?
ATP + D-alanine
-
Thermotoga maritima ADP + phosphate + D-alanyl-D-alanine
-
?
ATP + D-Arg at 60°C activity is 0.43% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Arg-D-Arg
-
?
ATP + D-Asn at 60°C activity is 0.22% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Asn-D-Asn
-
?
ATP + D-Asp at 60°C activity is 0.051% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Asp-D-Asp
-
?
ATP + D-Cys at 60°C activity is 29% of the activity with D-Ala Thermotoga maritima ADP + phosphate + D-Cys-D-Cys
-
?
ATP + D-Gln at 60°C activity is 0.56% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Gln-D-Gln
-
?
ATP + D-Glu at 60°C activity is 0.012% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Glu-D-Glu
-
?
ATP + D-His at 60°C activity is 0.49% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-His-D-His
-
?
ATP + D-Ile at 60°C activity is 0.36% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Ile-D-Ile
-
?
ATP + D-Leu at 60°C activity is 0.31% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Leu-D-Leu
-
?
ATP + D-Lys at 60°C activity is 0.6% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Lys-D-Lys
-
?
ATP + D-Met at 60°C activity is 0.34% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Met-D-Met
-
?
ATP + D-Phe at 60°C activity is 0.31% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Phe-D-Phe
-
?
ATP + D-Pro at 60°C activity is 0.29% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Pro-D-Pro
-
?
ATP + D-Ser at 60°C activity is 16% of the activity with D-Ala Thermotoga maritima ADP + phosphate + D-Ser-D-Ser
-
?
ATP + D-Thr at 60°C activity is 2.2% of the activity with D-Ala Thermotoga maritima ADP + phosphate + D-Thr-D-Thr
-
?
ATP + D-Trp at 60°C activity is 0.1% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Trp-D-Trp
-
?
ATP + D-Val at 60°C activity is 0.43% of the activity with D-Ala, no activity at 37°C Thermotoga maritima ADP + phosphate + D-Val-D-Val
-
?
ATP + Gly at 60°C activity is 3.6% of the activity with D-Ala Thermotoga maritima ADP + phosphate + Gly-Gly
-
?
beta-alanine + beta-alanine + ATP
-
Thermotoga maritima beta-alanyl-beta-alanine + ADP + phosphate 1.3% of the activity with D-serine ?
D-alanine + D-alanine + ATP
-
Thermotoga maritima D-alanyl-D-alanine + ADP + phosphate
-
?
D-cysteine + D-cysteine + ATP
-
Thermotoga maritima D-cysteinyl-D-cysteine + ADP + phosphate 29% of the activity with D-serine ?
D-serine + D-serine + ATP
-
Thermotoga maritima D-seryl-D-serine + ADP + phosphate 16% of the activity with D-serine ?
D-threonine + D-threonine + ATP
-
Thermotoga maritima D-threonyl-D-threonine + ADP + phosphate 2.2% of the activity with D-serine ?
glycine + D-alanine + ATP
-
Thermotoga maritima glycyl-D-alanine + ADP + phosphate 3.6% of the activity with D-serine ?
additional information substrate specificity expands at high temperature. At 37°C, enzyme shows reactivity only for D-alanine, D-serine, D-threonine, D-cysteine and glycine. At 60°C, substrates additionally include D-lysine, D-glutamine, D-histidine, D-arginine, D-valine, D-isoleucine, D-methionine, D-leucine, D-phenylalanine, D-proline, and D-asparagine, all below 1% of the activity with D-alanine Thermotoga maritima ?
-
?

Synonyms

Synonyms Comment Organism
Ddl
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 70% of maximal activity, 70°C: about 50% of maximal activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 90
-
Thermotoga maritima
90
-
15 min, stable up to Thermotoga maritima