Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no change of CofE activity is observed when the enzyme is assayed with the addition of 10 mM dithiothreitol to the reaction mixture | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta,gamma-CH2-GTP | 5 mM, 56% inhibition | Methanocaldococcus jannaschii | |
Ca2+ | 10 mM, only 26% F420-0 is converted to F420-1 | Methanocaldococcus jannaschii | |
Cs2+ | in the presence of either Rb+ or Cs+ at 0.2 M concentration, the only product of reaction is F420-1 | Methanocaldococcus jannaschii | |
GDP | 5 mM, 67% inhibition | Methanocaldococcus jannaschii | |
additional information | no significant inhibition when CofE is incubated with the following compound (10 mM): L-aspartate, Lglutamine, L-homocysteic acid, or DL-amino-4-phosphono-butyric acid | Methanocaldococcus jannaschii | |
Rb+ | in the presence of either Rb+ or Cs+ at 0.2 M concentration, the only product of reaction is F420-1 | Methanocaldococcus jannaschii | |
Zn2+ | 10 mM, only 26% F420-0 is converted to F420-1 | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
coenzyme gamma-F420-0 | pH 8.5, 50°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2 | Methanocaldococcus jannaschii | |
K+ | CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, with maximum stimulation occurring at 0.2 M KCl, NH4+ stimulates activity to a lesser extent, extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective | Methanocaldococcus jannaschii | |
Mg2+ | divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity | Methanocaldococcus jannaschii | |
Mn2+ | divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity | Methanocaldococcus jannaschii | |
NH4+ | CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, NH4+ stimulates activity to a lesser extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27150 | - |
2 * 27150, calculated from sequence | Methanocaldococcus jannaschii |
52100 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + GTP + coenzyme F420-0 | Methanocaldococcus jannaschii | step in the biosynthesis of coenzyme F420 | GDP + phosphate + coenzyme gamma-F420-1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58178 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + ATP + coenzyme F420-0 | - |
Methanocaldococcus jannaschii | ADP + phosphate + coenzyme F420-1 | - |
? | |
L-glutamate + dGTP + coenzyme F420-0 | coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 | Methanocaldococcus jannaschii | dGDP + phosphate + coenzyme F420-1 | - |
? | |
L-glutamate + GTP + coenzyme F420-0 | step in the biosynthesis of coenzyme F420 | Methanocaldococcus jannaschii | GDP + phosphate + coenzyme gamma-F420-1 | - |
? | |
L-glutamate + GTP + coenzyme F420-0 | coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 | Methanocaldococcus jannaschii | GDP + phosphate + coenzyme F420-1 | - |
? | |
L-glutamate + UTP + coenzyme F420-0 | coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 | Methanocaldococcus jannaschii | UDP + phosphate + coenzyme F420-1 | - |
? | |
additional information | CofE incubated with 10 mM beta-glutamate, D-glutamate, gamma-glutamylglutamate, DL-2-amino-3-phosphonopropionic acid, 2-carboxyethylphosphonic acid, or L-R-aminoadipic acid produces no F420-1 | Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27150, calculated from sequence | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
CofE | - |
Methanocaldococcus jannaschii |
F420-0:gamma-glutamyl ligase | - |
Methanocaldococcus jannaschii |
MJ0768 | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 80 | 35°C: about 30% of maximal activity, 80°C: about 55% of maximal activity | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
15 min, 30% loss of activity | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
with 50 mM CHES/Na+ buffer | Methanocaldococcus jannaschii |