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Literature summary for 6.3.2.31 extracted from

  • Li, H.; Graupner, M.; Xu, H.; White, R.H.
    CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii (2003), Biochemistry, 42, 9771-9778.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no change of CofE activity is observed when the enzyme is assayed with the addition of 10 mM dithiothreitol to the reaction mixture Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
beta,gamma-CH2-GTP 5 mM, 56% inhibition Methanocaldococcus jannaschii
Ca2+ 10 mM, only 26% F420-0 is converted to F420-1 Methanocaldococcus jannaschii
Cs2+ in the presence of either Rb+ or Cs+ at 0.2 M concentration, the only product of reaction is F420-1 Methanocaldococcus jannaschii
GDP 5 mM, 67% inhibition Methanocaldococcus jannaschii
additional information no significant inhibition when CofE is incubated with the following compound (10 mM): L-aspartate, Lglutamine, L-homocysteic acid, or DL-amino-4-phosphono-butyric acid Methanocaldococcus jannaschii
Rb+ in the presence of either Rb+ or Cs+ at 0.2 M concentration, the only product of reaction is F420-1 Methanocaldococcus jannaschii
Zn2+ 10 mM, only 26% F420-0 is converted to F420-1 Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
coenzyme gamma-F420-0 pH 8.5, 50°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2 Methanocaldococcus jannaschii
K+ CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, with maximum stimulation occurring at 0.2 M KCl, NH4+ stimulates activity to a lesser extent, extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective Methanocaldococcus jannaschii
Mg2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity Methanocaldococcus jannaschii
Mn2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity Methanocaldococcus jannaschii
NH4+ CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, NH4+ stimulates activity to a lesser extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27150
-
2 * 27150, calculated from sequence Methanocaldococcus jannaschii
52100
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + GTP + coenzyme F420-0 Methanocaldococcus jannaschii step in the biosynthesis of coenzyme F420 GDP + phosphate + coenzyme gamma-F420-1
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?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58178
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + ATP + coenzyme F420-0
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Methanocaldococcus jannaschii ADP + phosphate + coenzyme F420-1
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?
L-glutamate + dGTP + coenzyme F420-0 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 Methanocaldococcus jannaschii dGDP + phosphate + coenzyme F420-1
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?
L-glutamate + GTP + coenzyme F420-0 step in the biosynthesis of coenzyme F420 Methanocaldococcus jannaschii GDP + phosphate + coenzyme gamma-F420-1
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?
L-glutamate + GTP + coenzyme F420-0 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 Methanocaldococcus jannaschii GDP + phosphate + coenzyme F420-1
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?
L-glutamate + UTP + coenzyme F420-0 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 Methanocaldococcus jannaschii UDP + phosphate + coenzyme F420-1
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?
additional information CofE incubated with 10 mM beta-glutamate, D-glutamate, gamma-glutamylglutamate, DL-2-amino-3-phosphonopropionic acid, 2-carboxyethylphosphonic acid, or L-R-aminoadipic acid produces no F420-1 Methanocaldococcus jannaschii ?
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?

Subunits

Subunits Comment Organism
homodimer 2 * 27150, calculated from sequence Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
CofE
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Methanocaldococcus jannaschii
F420-0:gamma-glutamyl ligase
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Methanocaldococcus jannaschii
MJ0768
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Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 80 35°C: about 30% of maximal activity, 80°C: about 55% of maximal activity Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
15 min, 30% loss of activity Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
with 50 mM CHES/Na+ buffer Methanocaldococcus jannaschii