Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoate | 1 mM | Streptomyces clavuligerus | |
AMP | 5 mM | Streptomyces clavuligerus | |
ATP | at concentrations about 5 mM | Streptomyces clavuligerus | |
diphosphate | 5 mM | Streptomyces clavuligerus | |
EDTA | 10 mM | Streptomyces clavuligerus | |
iodoacetamide | 1 mM | Streptomyces clavuligerus | |
N-ethylmaleimide | 1 mM | Streptomyces clavuligerus | |
pyridoxal 5'-phosphate | 2 mM | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-cysteine | - |
Streptomyces clavuligerus | |
0.3 | - |
L-valine | - |
Streptomyces clavuligerus | |
0.63 | - |
L-aminoadipate | - |
Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration: 10 mM | Streptomyces clavuligerus |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | 8 mM leads to a 10% higher enzyme activity than at 3 mM | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0113 | - |
- |
Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminoadipate + L-cysteine + L-allo-isoleucine + ATP | - |
Streptomyces clavuligerus | L-delta-(aminoadipyl)-L-cysteinyl-D-allo-isoleucine + AMP + diphosphate | - |
? | |
L-2-aminoadipate + L-cysteine + L-isoleucine + ATP | - |
Streptomyces clavuligerus | L-delta-(aminoadipyl)-L-cysteinyl-D-isoleucine + AMP + diphosphate | - |
? | |
L-2-aminoadipate + L-cysteine + L-valine + ATP | - |
Streptomyces clavuligerus | N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + AMP + diphosphate | - |
ir | |
L-2-aminoadipate + L-homocysteine + L-valine + ATP | - |
Streptomyces clavuligerus | N-(5-amino-5-carboxypentanoyl)-L-homocysteinyl-D-valine + AMP + diphosphate | - |
? | |
L-glutamate + L-cysteine + L-valine + ATP | - |
Streptomyces clavuligerus | L-glutamyl-L-cysteinyl-D-valine + AMP + diphosphate | - |
? | |
S-carboxymethylcysteine + L-cysteine + L-valine + ATP | - |
Streptomyces clavuligerus | L-S-carboxymethylcysteinyl-L-cysteinyl-D-valine + AMP + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
29.5 | - |
- |
Streptomyces clavuligerus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 32 | - |
Streptomyces clavuligerus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half of activity after 70 h | Streptomyces clavuligerus |
25 | - |
half of activity after 10 h | Streptomyces clavuligerus |
32 | - |
half of activity after 10 min | Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Streptomyces clavuligerus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Streptomyces clavuligerus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
very unstable below pH 7 | Streptomyces clavuligerus |
8.5 | - |
very unstable above pH 8.5 | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | optimal concentration: 1.5-5 mM | Streptomyces clavuligerus |