KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-2-aminobutyrate | 37°C | Rattus norvegicus | |
1.4 | - |
L-2-aminobutyrate | 37°C | Homo sapiens | |
2.3 | - |
L-glutamate | 37°C | Rattus norvegicus | |
2.4 | - |
L-glutamate | 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | Rattus norvegicus | rate-limiting enzyme in glutathione synthesis | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | Homo sapiens | rate-limiting enzyme in glutathione synthesis | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P48506 | - |
- |
Rattus norvegicus | P19468 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Rattus norvegicus | - |
fibroblast | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
kinetic measurement, based on an HPLCESI-MS technique: L-2-aminobutyrate is used instead of cysteine as triggering substrate with saturating concentrations of glutamate and ATP, and the gamma-glutamylaminobutyrate formed is measured at m /z = 233 at regular time intervals. The reaction rate is maximum because ATP is held constant by enzymatic recycling of ADP by pyruvate kinase and phosphoenolpyruvate | Rattus norvegicus |
additional information | - |
kinetic measurement, based on an HPLCESI-MS technique: L-2-aminobutyrate is used instead of cysteine as triggering substrate with saturating concentrations of glutamate and ATP, and the gamma-glutamylaminobutyrate formed is measured at m /z = 233 at regular time intervals. The reaction rate is maximum because ATP is held constant by enzymatic recycling of ADP by pyruvate kinase and phosphoenolpyruvate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Glu + L-2-aminobutyrate | - |
Rattus norvegicus | ADP + phosphate + gamma-D-Glu-L-alpha-aminobutyrate | - |
? | |
ATP + D-Glu + L-2-aminobutyrate | - |
Homo sapiens | ADP + phosphate + gamma-D-Glu-L-alpha-aminobutyrate | - |
? | |
ATP + L-glutamate + L-cysteine | - |
Rattus norvegicus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | - |
Homo sapiens | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | rate-limiting enzyme in glutathione synthesis | Rattus norvegicus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | rate-limiting enzyme in glutathione synthesis | Homo sapiens | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamylcysteine ligase | - |
Rattus norvegicus |
gamma-glutamylcysteine ligase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Rattus norvegicus |
8.6 | - |
- |
Homo sapiens |