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Literature summary for 6.3.2.2 extracted from
- CYP2E1 overexpression in HepG2 cells induces glutathione synthesis by transcriptional activation of gamma-glutamylcysteine synthetase (2000), J. Biol. Chem., 275, 15563-15571.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | 40-50% increase in intracellular concentration induces enzyme expression 2fold to upregulate GSH production, GSH is required for detoxification, increase is reversible or preventable by peroxide-eliminating substances | Homo sapiens |  |
| additional information | no induction of enzyme production in HepG2 cells by overexpression of human cytochrome 3A4 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | Homo sapiens | rate-limiting enzyme in the GSH biosynthesis | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| additional information | Homo sapiens | overexpression of cytochrome P450 2E1 in human hepatocarcinoma cell line HepG2 increases the intracellular H2O2 level by 40-50% and therefore results in a 2fold increase in enzyme expression | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | - |
Homo sapiens | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-Cys | rate-limiting enzyme in the GSH biosynthesis | Homo sapiens | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| additional information | overexpression of cytochrome P450 2E1 in human hepatocarcinoma cell line HepG2 increases the intracellular H2O2 level by 40-50% and therefore results in a 2fold increase in enzyme expression | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-GCS | - |
Homo sapiens |
| gamma-Glutamylcysteine synthetase | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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