Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Myhrstad, M.C.; Carlsen, H.; Nordstrom, O.; Blomhoff, R.; Moskaug, J.O.
    Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter (2002), Free Radic. Biol. Med., 32, 386-393.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
apigenin nearly 2fold induction of the heavy subunit gene promotor and heavy subunit expression Homo sapiens
kaempferol 2fold induction of the heavy subunit gene promotor and heavy subunit expression Homo sapiens
additional information no increase in gene promotor activity by myricetin and sugar conjugates of quercetin, quercetin-3-glucoside and quercetin-3-rhamnoglucoside Homo sapiens
onion extract containing flavonoids, which increase the expression of both subunits of the enzyme in COS-1 cells Homo sapiens
quercetin 3fold induction of the heavy subunit gene promotor and heavy subunit expression, best at 0.05 mM, induction even of a distal part of the promotor sequence containing only 2 antioxidant-response/electrophile-response elements, i.e. ARE/EpRE Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of a construct consisting of 3.8 kb of the enzyme's heavy subunit gene promotor in front of luciferase as well as a fragment of the 5'-flanking sequence, transient expression in COS-1 cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-Cys
-
Homo sapiens ADP + phosphate + gamma-L-Glu-L-Cys
-
?

Subunits

Subunits Comment Organism
dimer heavy and light subunit Homo sapiens

Synonyms

Synonyms Comment Organism
gamma-glutaminylcysteine synthetase
-
Homo sapiens
GCS
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens