Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Hell, R.; Bergmann, L.
    gamma-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localization (1990), Planta, 180, 603-612.
    View publication on PubMed
Search for more literature for 6.3.2.2

General Stability

General Stability Organism
freezing, -20°C, and thawing results in 70-80% loss of activity Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 0.2 mM, activity is reduced by 35% Nicotiana tabacum
dithioerythritol
-
 Nicotiana tabacum
methionine sulfoximine
-
 Nicotiana tabacum
methionine sulfoximine
-
 Pisum sativum
Zn2+ 0.2 mM, activity is reduced by 19% Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 L-Cys
-
 Nicotiana tabacum
6.36
-
 2-aminobutyrate
-
 Nicotiana tabacum
10.4
-
 2-aminobutyrate L-Glu Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
chloroplast
-
 Pisum sativum 9507
-
cytoplasm
-
 Spinacia oleracea 5737
-
cytoplasm
-
 Pisum sativum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement, maximal activity at 30-50 mM Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Nicotiana tabacum
60000
-
 gel filtration Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-
Pisum sativum
-
-
-
Spinacia oleracea
-
-
-
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Nicotiana tabacum
-

Storage Stability

Storage Stability Organism
4°C, more than 50% loss of activity after 48 h. Stable at -20°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-methyl-L-glutamate + 2-aminobutyrate
-
 Nicotiana tabacum ADP + phosphate + gamma-L-glutamyl-2-aminobutyrate
-
 ?
ATP + L-Glu + L-Cys
-
 Triticum aestivum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Spinacia oleracea ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Pisum sativum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Nicotiana tabacum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Nicotiana tabacum

pH Range

pH Minimum pH Maximum Comment Organism
7 9 50% of maximal activity at pH 7.0 and pH 9.0 Nicotiana tabacum