Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | whole enzyme and large subunit inhibited | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27700 | - |
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
73000 | - |
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4°C, 10 mM imidazole buffer, pH 8.4, 50 mM DTT, irreversible loss of activity after 4 h | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
More | the heavy subunit contains all of the structural requirements for enzymatic activity and also for feedback inhibition by glutathione | Rattus norvegicus |