Cloned (Comment) | Organism |
---|---|
expression of wild type and mutant enzymes in AUXB1 cell line, which does not express measurable enzyme activity and is auxotrophic for the products of folate metabolism | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
A337V | L44 cells | Mus musculus |
G178E | L7 cells | Mus musculus |
G320D | L15 cells | Mus musculus |
S180F | L51 cells | Mus musculus |
S67F | L51 cells | Mus musculus |
S67F | L7 cells | Mus musculus |
T339I | L44 cells | Mus musculus |
W445stop | L15 cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,10-dideaza-5,6,7,8-tetrahydrofolic acid | lometrexo, used to generate the 5,10-dideazatetrahydrofolate resistant cells sublines | Mus musculus | |
methotrexate | the level required to inhibit the 5,10-dideazatetrahydrofolate resistant cell sublines is unchanged or slightly decreased compared with wild type cells | Mus musculus | |
N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid | raltitrexed, no inhibition observed with the 5,10-dideazatetrahydrofolate resistant cells sublines | Mus musculus | |
pemetrexed disodium | ALIMTA, no inhibition observed with the 5,10-dideazatetrahydrofolate resistant cells sublines | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
L-1210 cell | leukemia line, wild type and L7, L15, L44 and L51 5,10-dideazatetrahydrofolate resistant cells sublines | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000006 | - |
enzyme from L44 cells | Mus musculus |
0.0000001 | - |
enzyme from L15 cells | Mus musculus |
0.0000001 | - |
enzyme from L51 cells | Mus musculus |
0.0000001 | - |
enzyme from L7 cells | Mus musculus |
0.000008 | - |
wild type enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate | - |
Mus musculus | ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate | - |
? | |
ATP + 5-formyl-5,6,7,8-tetrahydrofolate + L-glutamate | - |
Mus musculus | ADP + 5-formyl-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate | - |
? | |
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate | folic acid | Mus musculus | ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2 | - |
? |