Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of methionine auxotrophs mutants by disruption of met7 gene, which encodes the enzyme | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
differential centrifugation and ultracentrifugation on Nycodenz gradients, partial purification | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000001 | - |
YK01 methionine auxotoph mutant, 5,10-methylene-tetrahydrofolate as a substrate | Saccharomyces cerevisiae |
0.0000015 | - |
YK01 methionine auxotoph mutant, tetrahydrofolate as a substrate | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | met7 mutant strain is a methionine auxotroph and an adenine and thymidine auxotroph when grown in the presence of sulfanilamide. Met7 mutants have a petite phenotype, defective in mitochondrial respiration and unable to grow on glycerol or other nonfermentable substrates as a carbon/energy source. Disruption of met7 causes deficiencies in folate metabolism and loss of both cytoplasmic and mitochondrial enzyme activity. Met7 mutants are complemented by genes that express only cytoplasmic enzyme | Saccharomyces cerevisiae | ? | - |
? |