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Literature summary for 6.3.2.17 extracted from

  • DeSouza, L.; Shen, Y.; Bognar, A.L.
    Disruption of cytoplasmic and mitochondrial folylpolyglutamate synthetase activity in Saccharomyces cerevisiae (2000), Arch. Biochem. Biophys., 376, 299-312.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information generation of methionine auxotrophs mutants by disruption of met7 gene, which encodes the enzyme Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
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Saccharomyces cerevisiae 5829
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mitochondrion
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Saccharomyces cerevisiae 5739
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-

Purification (Commentary)

Purification (Comment) Organism
differential centrifugation and ultracentrifugation on Nycodenz gradients, partial purification Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000001
-
YK01 methionine auxotoph mutant, 5,10-methylene-tetrahydrofolate as a substrate Saccharomyces cerevisiae
0.0000015
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YK01 methionine auxotoph mutant, tetrahydrofolate as a substrate Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information met7 mutant strain is a methionine auxotroph and an adenine and thymidine auxotroph when grown in the presence of sulfanilamide. Met7 mutants have a petite phenotype, defective in mitochondrial respiration and unable to grow on glycerol or other nonfermentable substrates as a carbon/energy source. Disruption of met7 causes deficiencies in folate metabolism and loss of both cytoplasmic and mitochondrial enzyme activity. Met7 mutants are complemented by genes that express only cytoplasmic enzyme Saccharomyces cerevisiae ?
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