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Literature summary for 6.3.2.17 extracted from

  • McGuire, J.J.; Hart, B.P.; Haile, W.H.; Rhee, M.S.; Galivan, J.; Coward, J.K.
    DL-beta,beta-Difluoroglutamic acid mediates position-dependent enhancement or termination of pteroylpoly(glutamate)synthesis catalyzed by folylpolyglutamate synthetase (1995), Arch. Biochem. Biophys., 321, 319-328.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3,3-difluoroglutamate i.e. beta,beta-difluoroglutamate??, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked Homo sapiens
3,3-difluoroglutamate i.e. beta,beta-difluoroglutamate??, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3,3-Difluoroglutamic acid i.e. beta,beta-difluoroglutamate, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked Homo sapiens
3,3-Difluoroglutamic acid i.e. beta,beta-difluoroglutamate, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
5,6,7,8-tetrahydropteroyl-Glu4 7,8-dihydropteroyl-Glu Homo sapiens
0.0031
-
3,3-difluoromethotrexate
-
Rattus norvegicus
0.004
-
aminopterin
-
Homo sapiens
0.0054
-
3,3-difluoromethotrexate
-
Homo sapiens
0.019
-
methotrexate
-
Homo sapiens
0.025
-
aminopterin pteroyl-3,3-difluoroglutamate Homo sapiens
0.047
-
methotrexate
-
Homo sapiens
0.132
-
pteroyl-Glu
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CEM cell
-
Homo sapiens
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3,3-difluoromethotrexate + L-glutamate
-
Homo sapiens ?
-
?
ATP + 3,3-difluoromethotrexate + L-glutamate
-
Rattus norvegicus ?
-
?
ATP + 3,5-difluoromethotrexate + Glu
-
Homo sapiens ADP + phosphate + 3,5-difluoromethotrexyl-Glu
-
?
ATP + 3,5-difluoromethotrexate + Glu
-
Rattus norvegicus ADP + phosphate + 3,5-difluoromethotrexyl-Glu
-
?
ATP + 3,5-difluoropteroyl-Glu + Glu
-
Homo sapiens ADP + phosphate + 3,5-difluoropteroyl-Glu2
-
?
ATP + 3,5-difluoropteroyl-Glu + Glu
-
Rattus norvegicus ADP + phosphate + 3,5-difluoropteroyl-Glu2
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Homo sapiens ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Rattus norvegicus ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
ATP + aminopterin + Glu
-
Homo sapiens ADP + phosphate + aminopteryl-Glu
-
?
ATP + aminopterin + Glu
-
Rattus norvegicus ADP + phosphate + aminopteryl-Glu
-
?
ATP + methotrexate + 3,3-difluoroglutamate
-
Rattus norvegicus ADP + phosphate + methotrexyl-3,3-difluoroglutamate
-
?
ATP + methotrexate + Glu
-
Homo sapiens ADP + phosphate + methotrexyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Homo sapiens ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Rattus norvegicus ADP + phosphate + pteroylmonoglutamyl-Glu
-
?