Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3,3-difluoroglutamate | i.e. beta,beta-difluoroglutamate??, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked | Homo sapiens | |
3,3-difluoroglutamate | i.e. beta,beta-difluoroglutamate??, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3-Difluoroglutamic acid | i.e. beta,beta-difluoroglutamate, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked | Homo sapiens | |
3,3-Difluoroglutamic acid | i.e. beta,beta-difluoroglutamate, , the effect on polyglutamylation is dependent on its position relative to the point of L-Glu ligation. When beta,beta-difluoroglutamate is the acceptor amino acid, i.e. point of attachment. Ligation of Glu is enhanced. When beta,beta-difluoroglutamate is one residue distal to the acceptor amino acid, further elongation is blocked | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
5,6,7,8-tetrahydropteroyl-Glu4 | 7,8-dihydropteroyl-Glu | Homo sapiens | |
0.0031 | - |
3,3-difluoromethotrexate | - |
Rattus norvegicus | |
0.004 | - |
aminopterin | - |
Homo sapiens | |
0.0054 | - |
3,3-difluoromethotrexate | - |
Homo sapiens | |
0.019 | - |
methotrexate | - |
Homo sapiens | |
0.025 | - |
aminopterin | pteroyl-3,3-difluoroglutamate | Homo sapiens | |
0.047 | - |
methotrexate | - |
Homo sapiens | |
0.132 | - |
pteroyl-Glu | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CEM cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3,3-difluoromethotrexate + L-glutamate | - |
Homo sapiens | ? | - |
? | |
ATP + 3,3-difluoromethotrexate + L-glutamate | - |
Rattus norvegicus | ? | - |
? | |
ATP + 3,5-difluoromethotrexate + Glu | - |
Homo sapiens | ADP + phosphate + 3,5-difluoromethotrexyl-Glu | - |
? | |
ATP + 3,5-difluoromethotrexate + Glu | - |
Rattus norvegicus | ADP + phosphate + 3,5-difluoromethotrexyl-Glu | - |
? | |
ATP + 3,5-difluoropteroyl-Glu + Glu | - |
Homo sapiens | ADP + phosphate + 3,5-difluoropteroyl-Glu2 | - |
? | |
ATP + 3,5-difluoropteroyl-Glu + Glu | - |
Rattus norvegicus | ADP + phosphate + 3,5-difluoropteroyl-Glu2 | - |
? | |
ATP + 7,8-dihydropteroyl-Glun + Glu | n: 1 | Homo sapiens | ADP + phosphate + 7,8-dihydropteroyl-Glun+1 | - |
? | |
ATP + 7,8-dihydropteroyl-Glun + Glu | n: 1 | Rattus norvegicus | ADP + phosphate + 7,8-dihydropteroyl-Glun+1 | - |
? | |
ATP + aminopterin + Glu | - |
Homo sapiens | ADP + phosphate + aminopteryl-Glu | - |
? | |
ATP + aminopterin + Glu | - |
Rattus norvegicus | ADP + phosphate + aminopteryl-Glu | - |
? | |
ATP + methotrexate + 3,3-difluoroglutamate | - |
Rattus norvegicus | ADP + phosphate + methotrexyl-3,3-difluoroglutamate | - |
? | |
ATP + methotrexate + Glu | - |
Homo sapiens | ADP + phosphate + methotrexyl-Glu | - |
? | |
ATP + pteroyl-Glu + Glu | n: 1, i.e. pteroylmonoglutamate, folic acid | Homo sapiens | ADP + phosphate + pteroylmonoglutamyl-Glu | - |
? | |
ATP + pteroyl-Glu + Glu | n: 1, i.e. pteroylmonoglutamate, folic acid | Rattus norvegicus | ADP + phosphate + pteroylmonoglutamyl-Glu | - |
? |