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Literature summary for 6.3.2.13 extracted from

  • Basavannacharya, C.; Robertson, G.; Munshi, T.; Keep, N.; Bhakta, S.
    ATP-dependent MurE ligase in Mycobacterium tuberculosis: Biochemical and structural characterisation (2010), Tuberculosis, 90, 16-24.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 3.0 A resolution in the presence of the substrate UDP-MurNAc-L-Ala-D-Glu. Protein consists of 3 domains consisting of residues 25-139,140-378 and 379-535, respectively. The UAG ligand binds to both domains 1 and 2 with the UDP portion binding to domain 1 and the peptide to domain 2. The substrate molecule UAG is bound between domains 1 and 2 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
ATP pH 7.4, 37°C Mycobacterium tuberculosis
0.04
-
UDP-N-acetylmuramoyl-L-Ala-D-Glu pH 7.4, 37°C Mycobacterium tuberculosis
0.069
-
meso-diaminopimelic acid pH 7.4, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WJL3
-
-
Mycobacterium tuberculosis H37Rv P9WJL3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid
-
Mycobacterium tuberculosis ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid
-
Mycobacterium tuberculosis H37Rv ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid
-
?
additional information highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity Mycobacterium tuberculosis ?
-
?
additional information highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity Mycobacterium tuberculosis H37Rv ?
-
?